Fig. 3
From: Biochemical characterization of type I-E anti-CRISPR proteins, AcrIE2 and AcrIE4
Test of the interaction between AcrIE2 and type I-E Cascade subunits. A Schematic representation of a type I-E CRISPR–Cas locus. Black diamonds and a red rectangle indicate invariable repeats and a variable phage-derived spacer, respectively. B Architecture of the type I-E Cascade. The complex has a subunit stoichiometry of Cas8e1:Cas112:Cas7e6:Cas5e1:Cas6e1:crRNA1. C Analytical SEC to evaluate interactions between AcrIE2 and individual Cas components of P. aeruginosa type I-E Cascade. AcrIE2 did not co-elute with any of the (His)6-MBP-tagged Cascade subunits. The elution fractions were analyzed by SDS-PAGE. The chromatogram and gel image for AcrIE2 alone are shown repeatedly with those for the five Cascade subunits as a control for comparison. Uncropped gel images are shown in Additional file 1: Figure S1