Fig. 4
From: Biochemical characterization of type I-E anti-CRISPR proteins, AcrIE2 and AcrIE4
AcrIE4 targets Cas8e in the type I-E CRISPR–Cas system. A Sequence alignment of AcrIE4 and AcrIE4-F7NTD. Conserved negatively charged residues, which were important for the interaction between AcrIE4-F7NTD and Cas8e in our previous study [26], are indicated with blue asterisks. B, C Analytical SEC experiments for interactions between AcrIE4 and Cas8e homologues. AcrIE4 co-eluted with P. aeruginosa Cas8e (B), but not with E. coli Cas8e (C). The elution fractions were analyzed by SDS-PAGE. Uncropped gel images are shown in Additional file 1: Figure S1. D ITC analysis of AcrIE4 binding to (His)6-MBP-tagged Cas8e of P. aeruginosa. The experimentally determined KD value is indicated with the fitting errors. Pa and Ec indicate P. aeruginosa and E. coli, respectively