Identification of compounds using HPLC-QTOF-MS online antioxidant activity mapping from aerial parts of Ligularia stenocephala

Applied Biological Chemistry

Table 1 DPPH radical scavenging activity of extract and fractions from L. stenocephala

Sample	Concentrations	DPPH radical scavenging activity (%)
l-Ascorbic acid	50 µM	61.9 ± 1.3^**
80% MeOH extract	1 µg/mL	1.0 ± 3.2
	5 µg/mL	7.6 ± 31.4
	10 µg/mL	6.1 ± 1.2
	30 µg/mL	27.6 ± 1.1^*
n-Hexane fraction	1 µg/mL	3.3 ± 1.8
	5 µg/mL	5.2 ± 0.2
	10 µg/mL	6.4 ± 1.7
	30 µg/mL	13.3 ± 0.6
EtOAc fraction	1 µg/mL
	5 µg/mL	7.4 ± 0.2^*
	10 µg/mL	22.8 ± 1.1^**
	30 µg/mL	35.9 ± 1.7^*
n-BuOH fraction	1 µg/mL	4.8 ± 0.3
	5 µg/mL	11.4 ± 1.2
	10 µg/mL	20.7 ± 0.4^**
	30 µg/mL	50.6 ± 0.9^***
Water fraction	1 µg/mL	3.1 ± 0.1
	5 µg/mL	5.2 ± 0.7
	10 µg/mL	4.2 ± 0.6
	30 µg/mL	19.7 ± 1.0^**

The experiment was repeated three times
^*p < 0.05, ^**p < 0.01, ^***p < 0.001 compared to control. l-Ascorbic acid was used as the positive control