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Table 1 Process parameter levels that were utilised in the experimental design

From: Optimisation of culture conditions for gesho (Rhamnus prinoides.L) callus differentiation using Artificial Neural Network-Genetic Algorithm (ANN-GA) Techniques

Independent variables

Levels

− 1

0

 + 1

Agar concentration (Y1, %)

0.3

0.5

0.9

Light duration (Y2, hours/day)

10

13

16

Culture temperature (Y3, °C)

16

28

40

Relative humidity (Y4, %)

55

70

100