Fig. 3

Effects of BFRG on the apoptosis in CT26 cells. A Cells were treated with BFRG at 0, 5, 10, 15 μg/mL for 24 h. Next, all samples were double stained with Annexin V-Alexa 647/PI and analyzed by flow cytometry. The upper right quadrant indicates late apoptosis, and the lower right quadrant indicates early apoptosis. The lower left quadrant illustrates live cells. B Quantification of the early, late and total apoptotic cells were shown. Data are presented as the mean of triplicate values \(\pm \) SD. *p < 0.05; ***p < 0.001; ****p < 0.0001