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Applied Biological Chemistry
Fig. 1 | Applied Biological Chemistry

Fig. 1

From: Wheat seedling extract and its constituents attenuate RANKL-induced differentiation and fusion of osteoclasts and bone resorption

Fig. 1

WSE inhibits RANKL-induced osteoclastogenesis. The BMMs were cultured for 4 d in the presence of RANKL (10 ng/mL) and M-CSF (30 ng/mL) in the presence of the vehicle (prethanol) or the indicated concentrations of WSE. A The cells were stained with TRAP solution. B TRAP-positive MNCs were counted as osteoclasts, and C TRAP activity was measured. D The cytotoxicity of WSE on BMMs was evaluated using the CCK-8 assay. Data are expressed as the mean ± SD and are representative of at least three experiments. * < 0.05; *** < 0.001 versus the RANKL-treated group; ### < 0.001 versus the control

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