Fig. 2

WSE inhibits c-Fos/NFATc1 transcription factors by attenuating AKT/ERK phosphorylation. A In the condition of serum starvation for 1 h, the BMMs were pretreated with or without WSE (3 µg/mL) for 1 h prior to RANKL stimulation (10 ng/mL) at the indicated time periods. Then, the protein expression levels were evaluated using western blot analysis, and one representative result obtained from three independent experiments yielding similar results is shown. B The BMMs were stimulated with RANKL (10 ng/mL) and M-CSF (30 ng/mL) in the presence or absence of WSE (3 µg/mL) for the indicated times. Total RNA was isolated using TRIzol reagent and the mRNA expression levels were evaluated using RT-qPCR. Relative levels of c-Fos, NFATc1, TRAP, and OSCAR were normalized to GAPDH. ^** < 0.01; ^*** < 0.0010 versus the vehicle control. C The effect of WSE on the protein expression level of RANKL-induced transcription factors was evaluated using western blot analysis. Actin was used as the internal control. Data are representative of at least three experiments