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Applied Biological Chemistry
Fig. 3 | Applied Biological Chemistry

Fig. 3

From: Wheat seedling extract and its constituents attenuate RANKL-induced differentiation and fusion of osteoclasts and bone resorption

Fig. 3

WSE impairs the fusion of preosteoclasts. A Based on the exposure schedule, the BMMs were cultured with WSE (3 µg/mL) for various times periods in the presence of M-CSF (30 ng/mL) and RANKL (10 ng/mL). B The cells were stained with TRAP solution. C TRAP activity was measured, and D TRAP-positive MNCs were counted as osteoclasts. Each exposure period of WSE was indicated as “0–4” for the vehicle. “0–1” for 0–1 d, “1–2” for 1–2 d, “2–3” for 2–3 d, and “3–4” for 3–4 d. ### < 0.001 versus the control; * < 0.05, ** < 0.01 versus the RANKL-treated group

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