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Applied Biological Chemistry
Fig. 4 | Applied Biological Chemistry

Fig. 4

From: Wheat seedling extract and its constituents attenuate RANKL-induced differentiation and fusion of osteoclasts and bone resorption

Fig. 4

WSE attenuates RANKL-induced actin ring formation. A Preosteoclasts incubated with or without 3 µg/mL WSE for 1 d. The formation of giant osteoclasts was observed using TRAP staining and fluorescence-conjugated phalloidin, and B TRAP-positive MNCs were counted as osteoclasts. C The effect of WSE on the mRNA expression of DC-STAMP was analyzed using RT-qPCR, and the relative-fold change of the mRNA expression level was normalized to GAPDH. ** < 0.01; *** < 0.001 versus the vehicle control. Data are representative of at least three experiments

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