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Applied Biological Chemistry
Fig. 5 | Applied Biological Chemistry

Fig. 5

From: Wheat seedling extract and its constituents attenuate RANKL-induced differentiation and fusion of osteoclasts and bone resorption

Fig. 5

WSE inhibits the function of mature osteoclasts. A The mature osteoclasts were plated on bone biomimetic synthetic surface and treated for 24 h with WSE (3 Âµg/ml). The cells stained with TRAP and TRAP + MNC formation was visualized under a light microscope (top images). The resorption areas formed by the cells were photographed under a light microscope (bottom images). B The resorptive areas (%) were quantified using the ImageJ software. ∗∗ < 0.01 versus the vehicle control. One representative result achieved from three independent experiments yielding similar results is shown. C The mRNA expression of Cathepsin K was evaluated during osteoclast differentiation in the absence or presence of WSE (3 Âµg/ml) using RT-qPCR and presented in comparison with the control. ** < 0.01; *** < 0.001 versus the vehicle control

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