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Applied Biological Chemistry
Fig. 6 | Applied Biological Chemistry

Fig. 6

From: Wheat seedling extract and its constituents attenuate RANKL-induced differentiation and fusion of osteoclasts and bone resorption

Fig. 6

Eight key compounds of WSE inhibits RANKL-induced osteoclastogenesis. A Typical chromatogram detected at 235 nm of phenolic phytochemical compositions in wheat seedlings extracts. The BMMs were cultured for 4 d in the presence of RANKL (10 ng/mL) and M-CSF (30 ng/mL) in the presence of the vehicle (DMSO) or the indicated concentrations of each compound. B The cells were stained with TRAP solution. C TRAP activity was measured. D The cytotoxicity of compounds on BMMs was evaluated using the CCK-8 assay. Data are expressed as the mean ± SD and are representative of at least three experiments. ### < 0.001 versus the negative control; *** < 0.001 versus the positive control

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