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Table 3 Calibration curves of phenolic acids and flavonoids

From: Antioxidative phenolic compounds from the aerial parts of Cyperus exaltatus var. iwasakii and their HPLC analysis

Compound

tR

Calibration Eq. a

Correlation factor, r2b

Gallic acid (1)

4.7

Y = 22.267X + 67.033

0.9992

Protocatechuic acid (2)

6.6

Y = 25.558X + 76.6

1.0000

Vanillic acid (3)

10.2

Y = 32.097X—129.03

0.9992

p-Coumaric acid (4)

17.3

Y = 33.68X + 128.23

0.9998

Rutin (5)

19.4

Y = 11.017X + 0.2

0.9999

Ferulic acid (6)

19.9

Y = 17.545X + 23.808

0.9999

Isoquercitrin (7)

22.1

Y = 19.87X + 269.63

0.9991

Astragalin (8)

26.2

Y = 23.083X + 73.537

0.9999

Quercetin (9)

40.4

Y = 35.876X + 41.054

1.0000

Luteolin (10)

41.0

Y = 30.651X—15.65

0.9996

Apigenin (11)

43.3

Y = 40.287 X + 72.525

1.0000

Tricin (12)

44.2

Y = 12.367 X + 168.19

0.9990

Kaempferol (13)

44.9

Y = 35.121X + 292.42

0.9999

  1. tR retention time
  2. aY = peak area, X = concentration of standard (µg/mL)
  3. br2 = correlation coefficient for five data points in the calibration curve