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Applied Biological Chemistry
Fig. 2 | Applied Biological Chemistry

Fig. 2

From: Development of multi-target dsRNAs targeting PcNLP gene family to suppress Phytophthora capsici infection in Nicotiana benthamiana

Fig. 2

Regulation of Phytophthora capsici infection and PcNLP2 expression via dsNLP2_MTF and dsNLP2_OReg. a The experimental scheme for dsRNA treatment and P. capsici infection. b The phenotype of P. capsici infection lesion introduced with mock and dsNLP2_MTF, respectively, in 3-week-old Nicotiana benthamiana leaves, determined using a FOBI in vivo fluorescence imaging system. Scale bar = 1 cm. c The phenotype of P. capsici infection lesion introduced with mock and dsNLP2_OReg, respectively. Scale bar = 1 cm. d Quantification of P. capsici infection lesion size using ImageJ. e Relative expression level of PcNLP2 using quantitative real-time PCR (qRT-PCR). Mock: Treatment with dsRNA targeting Renilla luciferase; dsNLP2_MTF: Treatment with dsNLP2_MTF; dsNLP2_OReg: Treatment with dsNLP2_OReg. MTF: Motif, OReg: Outer region. Data represent the mean ± standard error of mean (SEM; N = 3). Statistical significance is determined by Student’s t-test (*P < 0.05 and ***P < 0.001)

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