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Fig. 1 | Applied Biological Chemistry

Fig. 1

From: Hypothetical protein CuvA (Rv1422) from Mycobacterium tuberculosis H37Rv interacts with uridine diphosphate N-acetylglucosamine as a key precursor of cell wall

Fig. 1

Analysis of protein thermal shift in the presence of increasing concentrations (0.2–8 mM) of UDP-sugars. MtCuvA melting profiles were monitored in the presence of increasing concentrations of A UDP-Glc and B UDP-GlcNAc. The melting temperature (Tm) of protein is obtained at the midpoint of each melting curve. C Non-linear square-fit curve for the binding of UDP-sugars of MtCuvA. The plots represent the values of ΔTm for increasing concentrations of UDP-Glc and UDP-GlcNAc, respectively

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