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Applied Biological Chemistry
Fig. 2 | Applied Biological Chemistry

Fig. 2

From: Hypothetical protein CuvA (Rv1422) from Mycobacterium tuberculosis H37Rv interacts with uridine diphosphate N-acetylglucosamine as a key precursor of cell wall

Fig. 2

Identification of key residues of MtCuvA involved in the binding of UDP-GlcNAc. A Superimposition of a proposed homology model of MtCuvA with B. halodurans GlmR (PDB ID: 2O2Z). Differential conformation is shown in the ribbon shape colored in red. B Best-fit model by docking simulation of MtCuvA model with UDP-GlcNAc. Residues suggested to form the hydrogen bonds with the ligand are shown as sticks. The hydrogen bond interactions are indicated by black dotted lines with the distances. C Residues were predicted to interact with the ligand in the putative ligand-binding pocket of the MtCuvA model. Residues applied to the site-directed mutagenesis are shown as sticks and colored by element types. D–K Non-linear square-fit curve for the UDP-GlcNAc binding to MtCuvA mutants. The melting profiles of H12A (D), T33A (E), D36A (F), Q154A (G), S196A (H), T199A (I), N226A (J), and H298A (K) were monitored in the presence of increasing concentrations (0.2–8 mM) of UDP-GlcNAc

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