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Applied Biological Chemistry
Fig. 3 | Applied Biological Chemistry

Fig. 3

From: Hypothetical protein CuvA (Rv1422) from Mycobacterium tuberculosis H37Rv interacts with uridine diphosphate N-acetylglucosamine as a key precursor of cell wall

Fig. 3

Binding properties of MtCuvA with two moieties of UDP-GlcNAc. A Chemical structures of UDP-GlcNAc and its two moieties (UDP and GlcNAc). B The melting temperatures (Tm) of MtCuvA with or without UDP-GlcNAc, UDP, and GlcNAc (each 5 mM). Four replicate reactions were performed on a real-time PCR system using the melt curve by protein thermal shift. C Non-linear square-fit curve for the UDP and UDP-GlcNAc binding to MtCuvA using thermal shift assay. The melting profiles were monitored in the presence of increasing concentrations (0.2–8 mM) of ligands

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