Table 1 Oligonucleotide primers in forward (F) and reverse (R) directions for site-directed mutagenesis
Primers | Sequences (5′–3′) |
|---|---|
H12A-F | CGCGCTGGGCGGCGGAGCCGGCTTCTATGC |
H12A-R | CAGCGTCGCATACAAGCCGGCTCCGCCGCC |
T33A-F | GTTACCGCCGTGGTGGCGGTCGCCGATGAC |
T33A-R | GCCACCGTCATCGGCGTCCGCCACCACGGC |
D36A-F | GTGGTGACCGTCGCCGCGGACGGTGGCTCG |
D36A-R | GGCCCGACGAGCCACCGTCCGCGGCGACGG |
R80A-F | ACTATTCTGCAGCACGCGTTCGGCGGCAGT |
R80A-R | ACCACTGCCGCCGAACGCGTGCTGCAGATT |
Q154A-F | CGCCTGATCCGTGGCGCGGTGGCGATCGCG |
Q154A-R | GTGGTCGCGATCGCCACCGCGCCACGGATC |
S196A-F | GTCCTGGGGCCCGGGGCGTGGTTCACCAGC |
S196A-R | CACGCTGGTGAACCACGCCCCGGGCCCCAG |
T199A-F | CCCGGGTCGTGGTTCGCGAGCGTGATACCC |
T199A-R | ATGGGGTATCACGCTCGCGAACCACGACCC |
S200A-F | GGTCGTGGTTCACCGCGGTGATACCCCATG |
S200A-R | CCAGCACATGGGGTATCACCGCGGTGAACC |
N226A-F | CCGCCGTGCCCTGGTGCTCGCCCTGGTGGC |
N226A-R | CCCGGTTCAGCCACCAGGGCGAGCACCAGG |
E230A-F | GTGCTCAACCTGGTGGCTGCACCGGGAGAG |
E230A-R | CCGGCCGTCTCTCCCGGTGCAGCCACCAGG |
T295A-F | CGATGTCGCCAGACCTGGTGCACCTTTAC |
T295A-R | GCCCGGGTCATGTAAAGGTGCACCAGGTC |
H298A-F | CAGACCTGGTACACCTTTAGCTGACCCGGG |
H298A-R | CCGCCAGCTTGCCCGGGTCAGCTAAAGGTG |