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Applied Biological Chemistry

Table 2 Kinetic parameters for the binding of UDP-sugars to MtCuvA

From: Hypothetical protein CuvA (Rv1422) from Mycobacterium tuberculosis H37Rv interacts with uridine diphosphate N-acetylglucosamine as a key precursor of cell wall

Variable ligands

KD (mM)

ΔTm max

Binding Efficiency

(ΔTm max/KD)

R2

UDP-Glc

1.36 ± 0.36

3.64 ± 0.33

2.68 ± 0.75

0.966

UDP-GlcNAc

0.38 ± 0.06

9.47 ± 0.34

24.92 ± 4.04

0.986

  1. R2 = Coefficient of determination
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