Table 4 Kinetic parameters for the binding of UDP to MtCuvA mutants

Wild type

1.71 ± 0.11

3.06 ± 0.07

1.79 ± 0.12

0.998

H12A

1.85 ± 0.28

2.08 ± 0.12

1.12 ± 0.18

0.991

T33A

1.87 ± 0.13

4.69 ± 0.12

2.51 ± 0.19

0.998

D36A

1.57 ± 0.12

4.88 ± 0.13

3.11 ± 0.25

0.997

Q154A

1.55 ± 0.34

3.21 ± 0.25

2.07 ± 0.48

0.978

S196A

25.20 ± 23.15

6.02 ± 4.43

0.24 ± 0.28

0.974

T199A

5.51 ± 3.28

2.35 ± 0.75

0.43 ± 0.29

0.914

N226A

ND^*

ND

ND

–

H298A

ND^*

ND

ND

–