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Applied Biological Chemistry

Table 2 List of primer sequences used in real-time PCR

From: Centella asiatica lowers body fat accumulation via regulating cholesterol homeostasis- and lipid metabolism-related genes in mice with high-fat, high-sugar diet-induced obesity

Gene

Primer sequence (5ʹ-3ʹ)

Forward

Reverse

GAPDH

CATCACTGCCACCCAGAAGACTG

ATGCCAGTGAGCTTCCCGTTCAG

PPARγ

GTACTGTCGGTTTCAGAAGTGCC

ATCTCCGCCAACAGCTTCTCCT

FAS

CTGCGATTCTCCTGGCTGTGAA

CAACAACCATAGGCGATTTCTGG

CD36

GGACATTGAGATTCTTTTCCTCTG

GCAAAGGCATTGGCTGGAAGAAC

AMPK

GGTGTACGGAAGGCAAAATGGC

CAGGATTCTTCCTTCGTACACGC

SREBP-2

AGAAAGAGCGGTGGAGTCCTTG

GAACTGCTGGAGAATGGTGAGG

HMGCR

GCTCGTCTACAGAAACTCCACG

GCTTCAGCAGTGCTTTCTCCGT

SREBP-1 C

CGACTACATCCGCTTCTTGCAG

CCTCCATAGACACATCTGTGCC

LDLR

GAATCTACTGGTCCGACCTGTC

CTGTCCAGTAGATGTTGCGGTG

SCD1

GCAAGCTCTACACCTGCCTCTT

CGTGCCTTGTAAGTTCTGTGGC

  1. GAPDH glyceraldehyde-3-phosphate dehydrogenase, PPARγ peroxisome proliferator activated receptor gamma, FAS fatty acid synthase, CD36 cluster of differentiation 36, AMPK 5ʹadenosine monophosphate activated protein kinase, SREBP-2 sterol regulatory element binding protein 2, HMGCR 3-hydroxyl-3-methylglutaryl CoA reductase, SREBP-1C sterol regulatory element binding protein 1C, LDLR low-density lipoprotein receptor, SCD1 stearoyl CoA desaturase 1
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