Fig. 4

Tertiary structure prediction and subcellular localization in tobaccos of SlCPRs. A, Diagrams presenting the predicted tertiary structures of the two SlCPRs. The protein structures were predicted using SWISS-MODEL, with a rainbow coloring gradient from the N-terminal (blue) to the C-terminal (red). The predicted transmembrane sites are indicated using gray dots. B, Confocal microscopy images of N. benthamiana epidermal cells. A 35S promoter was used for all vectors, and CPRs were linked to the N-terminus of EGFP and expressed (in green). The endoplasmic reticulum was labeled in red. The EGFP and mCherry signals were individually captured through the green and red channels, respectively. Yellow in the overlay images indicates co-localization. Scale bar: 20 µm.