Skip to main content
Applied Biological Chemistry

Table 1 Kinetic parameters of MTT, ferricyanide, CTC, and cytochrome c reduction by the SlCPRs

From: Heterologous expression in E. coli and functional characterization of the tomato CPR enzymes

 

SlCPR1

SlCPR2

Kcat (s−1)

Km (μM)

Kcat/Km

Kcat (s−1)

Km (μM)

Kcat/Km

MTT

49.4 ± 2.1

26.3 ± 4.1

1.88

147 ± 2.5

13.7 ± 0.92

10.7

CTC

25.3 ± 0.64

20.6 ± 2.1

1.23

30.7 ± 0.95

22.7 ± 2.9

1.35

Ferricyanide

228 ± 6.5

117 ± 9.1

1.95

282 ± 5.6

21.2 ± 1.7

13.3

Cytochrome c

51.9 ± 2.1

24.2 ± 1.0*

2.14

91.8 ± 0.81

24.6 ± 0.23*

3.73

  1. Kinetic parameters were studied through the reaction of the two SICPRs with different concentrations of substrates (MTT, CTC, and ferricyanide, 0–500 μM; cytochrome c, 0–100 μM). The values for MTT, CTC, and ferricyanide were calculated through non-linear regression analysis of Michaelis–Menten plots and those for cytochrome c were calculated through asymmetric sigmoidal analysis, using the respective substrate coefficients. The values are presented as mean and standard errors of three repeats. *Shows K1/2 instead of Km
Back to article page