Biochemical characterization of a regiospecific flavonoid 3'-O-methyltransferase from orange

Applied Biological Chemistry

Table 2 Substrate preference of recombinant CsOMT16 expressed in E. coli

^aThe OMT activity toward flavonoids respective to that toward quercetin at a substrate concentration of 50 µM
^bMinus signs indicate that no product was detected in the HPLC analysis
^cPlus signs represent that the flavonoids tested were used as a substrate that yielded a product peak in the HPLC analysis. The relative activities of the substrates were not determined