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Table 3 Kinetic parameters of recombinant CsOMT16a

From: Biochemical characterization of a regiospecific flavonoid 3'-O-methyltransferase from orange

Substrate

Km

(μM)

Vmax

(pmol min−1 μg−1)

kcat

(min−1)

kcat/Km

(M−1 s−1)

Eriodictyol

2.62 ± 0.75

32.25 ± 3.81

1.36

8.66 × 103

Luteolin

1.17 ± 0.09

60.62 ± 9.28

2.55

3.65 × 104

Quercetin

4.52 ± 0.81

89.33 ± 5.36

3.76

1.39 × 104

Rhamnetin

0.25 ± 0.05

10.34 ± 0.32

0.44

2.91 × 104

  1. aThe results represent the mean and standard deviation from three independent experiments