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Applied Biological Chemistry
Fig. 1 | Applied Biological Chemistry

Fig. 1

From: Optimized protocols for protoplast isolation, transfection, and regeneration in the Solanum genus for the CRISPR/Cas-mediated transgene-free genome editing

Fig. 1

Schematic diagram of protoplast isolation from plant cotyledons. A Cotyledon preparation by slicing into thin strips (0.5 to 1 mm), B Incubation of sliced cotyledons in the digestion solution for 16 h, C Confirmation of the release of protoplasts in the digestion solution, D Filtration using a 40 µm mesh to remove cell debris, E Removal of the enzyme solution by washing with the stabilizing solution twice, F Microscopic observation to count protoplasts. Scale bar indicates 50 μm

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