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Applied Biological Chemistry
Fig. 3 | Applied Biological Chemistry

Fig. 3

From: Optimized protocols for protoplast isolation, transfection, and regeneration in the Solanum genus for the CRISPR/Cas-mediated transgene-free genome editing

Fig. 3

Plant regeneration from the tomato protoplasts. A Tomato protoplasts freshly isolated from cotyledons, B Protoplasts during first cell divisions in 7 days after isolation in the protoplast culture medium, C Protoplasts during second division after 14 days in the protoplast culture medium, D Cell aggregate formation after 3 weeks in the protoplast culture medium, E Callus formation after 1 months on the callus induction medium, F Callus growth for 2 months on the callus growth medium, G Shoot regenerated on the shoot induction medium for 2 months. Scale bars, 50 μm A–D and 1 cm E–G

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