Fig. 4

Effects of TP1 on the protein expression levels of pro-inflammatory enzymes. Raw264.7 macrophages were pretreated with TP1 (25 µM), followed by treatment with LPS (0.1 µg/mL) for 24 h. a Protein expression levels were measured using western blot analysis. b and c iNOS/β-actin and COX-2/β-actin expression levels were quantified by ImageJ software. *p < 0.05 compared with the LPS group. The data are presented as mean ± standard deviation (SD). Three independent experiments were performed. Among them, representative data are presented