Table 1 List of primers used for RT-PCR
Primer | Sequence | Tm value (℃) | Length (bp) |
|---|---|---|---|
Mo GAPDH forward | TCACTGCCACCCAGAAGAC | 58 | 450 |
Mo GAPDH reverse | TGTAGGCCATGAGGTCCAC | ||
Mo SOX9 forward | ATGCTATCTTCAAGGCGCTG | 60 | 272 |
Mo SOX9 reverse | GACGTCGAAGGTCTCAATGT | ||
Mo COL2A1 forward | CACACTGGTAAGTGGGGCAAGACCG | 58 | 173 |
Mo COL2A1 reverse | GGATTGTGTTGTTTCAGGGTTCGGG | ||
Mo aggrecan forward | GAAGACGACATCACCATCCAG | 60 | 581 |
Mo aggrecan reverse | CTGTCTTTGTCACCCACACAT | ||
Mo MMP3 forward | AGGGATGATGATGCTGGTATGG | 58 | 434 |
Mo MMP3 reverse | CCATGTTCTCCAACTGCAAAGG | ||
Mo MMP13 forward | TGATGGACCTTCTGGTCTTCTGG | 58 | 474 |
Mo MMP13 reverse | CATCCACATGGTTGGGAAGTTCT | ||
Mo ADMTs4 forward | CATCCGAAACCCTGTCAACTTG | 58 | 287 |
Mo ADAMTs4 reverse | GCCCATCATCTTCCACAATAGC | ||
Mo ADAMTs5 forward | GCCATTGTAATAACCCTGCACC | 58 | 292 |
Mo ADAMTs5 reverse | TCAGTCCCATCCGTAACCTTTG | ||
Mo MMP9 forward | TGCACTGGGCTTAGATCATTCC | 58 | 450 |
Mo MMP9 reverse | CCGTCCTTGAAGAAATGCAGAG | ||
Mo Col10a1 forward | GCCAGGTCTCAATGGTCCTA | 58 | 484 |
Mo Col10a1 reverse | AAAAGCAGACACGGGCATAC | ||
Mo Runx2 forward | GCCACCTTTACCTACACCCC | 60 | 117 |
Mo Runx2 reverse | ACTCTGGCTTTGGGAAGAGC |