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Applied Biological Chemistry
Fig. 5 | Applied Biological Chemistry

Fig. 5

From: Soybean flower-specific R2R3-MYB transcription factor gene GmMYB108 induces anthocyanin production in Arabidopsis thaliana

Fig. 5

Expression analysis of anthocyanin biosynthesis and regulatory genes in GmMYB108-OE transgenic Arabidopsis plants. The effect of GmMYB108 overexpression on endogenous biosynthetic and regulatory genes was investigated using qRT-PCR analysis in GmMYB108-OE transgenic Arabidopsis plants (T2 generation) (Additional file 1: Fig. S3). The relative expression levels (fold change) are depicted as mean ± SD of three biological replicates in triplicate. The protein phosphatase 2 regulatory subunit (PP2A) gene (At1g69960) was used as an endogenous reference to normalize gene expression. (A) Anthocyanin biosynthesis genes: chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), dihydroflavonol-4-reductase (DFR), anthocyanidin synthase (ANS), and UDP-glucose:flavonoid 3-O-glucosyltransferase (UF3GT). B GmMYB108, Anthocyanin-regulatory genes: MYB11, MYB12, MYB111, production of anthocyanin pigment 1 (PAP1), PAP2, MYB113, MYB114, transparent testa 8 (TT8), glabra 3 (GL3), enhancer of glabra 3 (EGL3), and MYB-like 2 (MYBL2)

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