Table 1 Proposed analytical method for determination of 16 phenolic compounds in G. littoralis
HPLC unit | Agilent 1260 series |
|---|---|
Column | YMC Hydrosphere C18 (4.6 × 250 mm, i.d., 5 µm particle size) |
Detector | Diode array detector (DAD) |
Detection wavelength (nm) | 254 nm |
Flow rate (mL min−1) | 1.0 mL min−1 |
Injection volume | 10 µL |
Column temperature | 35 ℃ |
Mobile phase | A: 0.3% formic acid in Water B: 0.3% formic acid in Acetonitrile C: Methanol |
Time (min) | Mobile phase A (%) | Mobile phase B (%) | Mobile phase C (%) |
|---|---|---|---|
HPLC gradient profile | |||
0 | 88 | 12 | 0 |
8 | 88 | 12 | 0 |
12 | 82 | 18 | 0 |
22 | 82 | 18 | 0 |
26 | 77 | 23 | 0 |
27 | 50 | 0 | 50 |
37 | 50 | 0 | 50 |
56 | 9 | 0 | 91 |
65 | 9 | 0 | 91 |