Development and application of a high-performance liquid chromatography diode-array detection (HPLC–DAD) method for the simultaneous quantification of phenolic compounds in the aerial part of Glehnia littoralis

Applied Biological Chemistry

Table 2 Results for the parameters of method validation of simultaneous determination for 16 phenolic compounds in G. littoralis by HPLC–DAD

Peak	Compound	Calibration range (μg mL⁻¹)	Calibration curve	Correlation coefficient (R²)	LOD (μg mL⁻¹)	LOQ (μg mL⁻¹)
1	Chlorogenic acid	5–1000	y = 33.176x + 22.421	0.9996	1.93	5.86
2	Caffeic acid	2.5–50	y = 54.162x − 11.175	0.9994	0.31	0.95
3	Scopoletin	2.5–50	y = 39.628x − 5.7914	0.9993	0.34	1.04
4	Rutin	5–1000	y = 20.75x − 50.604	0.9999	1.39	4.23
5	Hyperoside	2.5–50	y = 28.517x − 5.9583	0.9992	0.63	1.90
6	Isoquercetin	5–100	y = 45.605x − 21.084	0.9992	1.27	3.85
7	Nicotiflorin	5–100	y = 16.772x − 4.5759	0.9993	1.26	3.82
8	Psoralen	2.5–50	y = 84.526x − 25.319	0.9996	0.63	1.92
9	Xanthotoxin	2.5–50	y = 46.756x − 7.2072	0.9993	0.58	1.76
10	Isopimpinellin	2.5–50	y = 23.361x − 3.2631	0.9993	0.59	1.78
11	Bergapten	2.5–50	y = 47.866x − 12.234	0.9995	0.71	2.15
12	Oxypeucedanin	2.5–50	y = 30.84x − 2.5168	0.9993	0.69	2.08
13	Imperatorin	2.5–50	y = 41.322x − 34.968	0.9997	1.00	3.03
14	Phellopterin	2.5–50	y = 25.084x − 1.9087	0.9993	0.60	1.82
15	Isoimperatorin	2.5–50	y = 24.793x − 3.4156	0.9993	0.62	1.87
16	Bergamottin	2.5–50	y = 29.773x − 5.1171	0.9993	0.59	1.80