Development and application of a high-performance liquid chromatography diode-array detection (HPLC–DAD) method for the simultaneous quantification of phenolic compounds in the aerial part of Glehnia littoralis

Applied Biological Chemistry

Table 3 Contents (mg g⁻¹ dry weight) of 16 phenolic compounds in the G. littoralis aerial parts and roots

Classification	Peak	Compound	Aerial	Root
Coumarin	3	Scopoletin	0.01 ± 0.00^**	0.01 ± 0.00
	8	Psoralen	n.d.¹	n.d
	9	Xanthotoxin	0.05 ± 0.00^*	0.04 ± 0.00
	10	Isopimpinellin	0.16 ± 0.02^**	n.d
	11	Bergapten	0.14 ± 0.02^**	n.d
	12	Oxypeucedanin	0.02 ± 0.00^*	n.d
	13	Imperatorin	0.41 ± 0.01^***	0.02 ± 0.00
	14	Phellopterin	0.03 ± 0.01^***	n.d
	15	Isoimperatorin	0.09 ± 0.00^***	0.02 ± 0.00
	16	Bergamottin	n.d	n.d
		Total	0.93 ± 0.04^***	0.09 ± 0.01
Flavonoid	4	Rutin	10.01 ± 0.09^***	n.d
	5	Hyperoside	0.02 ± 0.01^*	n.d
	6	Isoquercetin	0.94 ± 0.01^***	n.d
	7	Nicotiflorin	2.19 ± 0.04^***	n.d
		Total	13.16 ± 0.11	n.d
Hydroxycinnamic acid	1	Chlorogenic acid	4.23 ± 0.02^***	0.10 ± 0.01
Hydroxycinnamic acid	2	Caffeic acid	0.02 ± 0.00^***	n.d
		Total	4.25 ± 0.02^***	0.10 ± 0.01

Each data was represented as mean ± standard deviation (n = 3) and statistically analyzed using one-tailed t-test. Significant differences were assessed by ***(P < 0.001), **(P < 0.01), *(P < 0.05)
¹n.d., not detected