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Applied Biological Chemistry
Fig. 2 | Applied Biological Chemistry

Fig. 2

From: Acetyl genistin modulates myotube differentiation and attenuates dexamethasone-induced muscle atrophy through the FoxO1/3 signaling pathway in C2C12 myotubes

Fig. 2

Acetyl genistin enhances the expression of MHC, MyoD and MyoG. a Quantitative evaluation of myogenic marker genes (MyoD and MyoG) over 3 time points (day 2, day 4, and day 6) in cells exposed to either the control or acetyl genistin treatment. Glyceraldehyde 3-phosphate dehydrogenase was used as a control. Data are shown as mean ± standard deviation (SD) (n = 4 per group). ** p < 0.01 and *** p < 0.001 vs. CTL. b The expression of MHC, MyoD and MyoG protein in C2C12 myotubes was estimated by western blot analysis. β-actin was used as a control for protein loading. Data are shown as mean ± SD. (n = 3 per group). * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. CTL. CTL control, AG acetyl genistin

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