Fig. 3

AG attenuates dexamethasone-induced muscle atrophy in C2C12 myotubes by suppressing MAFbx and MuRF1 expression. a May–Grunwald and Giemsa staining. The cells were incubated with 40 μM acetyl genistin for 48 h in the presence or absence of 1 μM dexamethasone for 48 h. b MAFbx and MuRF1 mRNA levels were analyzed by quantitative polymerase chain reaction. Glyceraldehyde 3-phosphate dehydrogenase was used as a control. Data are shown as mean ± standard deviation (SD) (n = 4 per group). ** p < 0.01 and *** p < 0.001 vs. CTL; ## p < 0.01 vs. Dexa. c The expression of MAFbx and MuRF1 protein in C2C12 myotubes was estimated by western blot analysis. β-actin was used as a control for protein loading. Data are shown as mean ± SD. (n = 3 per group). *** p < 0.001 vs. CTL, ## p < 0.01 vs. Dexa. CTL control, Dexa dexamethasone, AG acetyl genistin, DAG dexamethasone + acetyl genistin