Fig. 2
From: Piperine reduces hair oiliness by inhibiting adipogenesis of hair stem cells
Downregulated expression of adipogenic genes by piperine. (A) Effect of piperine on the expression of adipogenic genes. Total RNA from skin samples of mice fed the indicated diets was extracted and subjected to cDNA synthesis followed by qPCR. Pparγ, Fabp4, Lpl, and Adipoq: PPARγ target genes. Blimp1, Pparγ, and Fasn: SG markers. Gene expression was normalized to Gapdh, as the internal control. Results are means ± SD (n = 5, *p < 0.05, **p < 0.01, and ***p < 0.001). (B, C) Effect of piperine on the tissue expression of Pparγ (B) and Adipoq (C). Fluorescence immunohistochemistry was performed using skin tissues from the ND, HFD, and piperine-supplemented HFD groups. Scale bars, 75 μm. Dashed white lines, epidermal–dermal boundary