Fig. 3
From: Piperine reduces hair oiliness by inhibiting adipogenesis of hair stem cells
Antagonistic effect of piperine on the adipogenesis of BSCs. The data represent means ± SD (n = 3, *p < 0.05 and ***p < 0.001). (A) Effect of piperine on BSC viability. Cytotoxicity was determined by MTT assay. (B) Effect of piperine on Oil Red O (ORO) staining of BSCs. Adipogenesis was induced in adipogenic differentiation medium for 14 days. (C) Quantification of ORO staining. ORO-stained lipids were extracted using isopropanol and quantitated by measuring the absorbance at 500 nm using a spectrophotometer. (D) Downregulation of adipogenic genes by piperine. After induction of adipogenesis of BSGs by piperine, the expression of PPARγ target and SG marker genes was evaluated by RT-qPCR. C, culture medium; D, differentiation medium; P, piperine (100 µM)