Fig. 3

HYP restored the depression like behavior and the protein expression of nNOS, BDNF, and phosphorylated TrkB in OVX mice. (A) Experimental design and schedule. OVX mice were orally administered E2 (10 µg/kg) or HYP (30 mg/kg) for 24 weeks. (B) Depression-like behaviors were assessed with the open field test. The locomotor activity was analyzed by recording the total distance traveled in 5 min. (C) Western blot showing the protein expression of nNOS, BDNF and phosphorylated TrkB. Results are presented as mean ± SE. ##P < 0.01, ###P < 0.001 vs. Sham mice; *P < 0.05, ***P < 0.001vs. OVX mice. BDNF, brain-derived neurotrophic factor; E2, 17β-estradiol; HYP, hyperoside; nNOS, neuronal nitric oxide synthase; NO, nitric oxide; OFT, open field test; OVX, ovariectomized; TrkB, tropomyosin receptor kinase B