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Evaluation of the antioxidant activity of the fruiting body of Phellinus linteus using the on-line HPLC-DPPH method


Phellinus linteus has traditionally been used as a medicinal mushroom in Oriental countries. Bioactive phytochemicals from the fruiting body of P. Linteus were investigated using the on-line HPLC-DPPH system. The system was used to separate 10 antioxidants: protocatechuic acid (1) protocatechualdehyde, (2) caffeic acid (3), ellagic acid (4), hispidin (5), davalliallactone (6), interfungins A (7), hypholomine B (8), inoscavin A (9), and methyldavallialactone (10). Of these, compounds 6 and 7 showed the strongest inhibitory effect against the DPPH radical and superoxide anion radical-scavenging capacity. On comparison with quercetin (IC50, 44.0 μM), the IC50 value of compounds 6 and 7 for DPPH radical-scavenging capacity were 19.6 μM and 18.5 μM. Moreover, the antioxidative capacity of compound 6 at 40mM was 2.95 nmol Trolox equivalents per mg dry wt. These results indicated that hispidin dimers such as hispidin with hispidin or hispolon had a stronger effect on radical-scavenging capacity than hispidin itself.





electospray ionization


heteronuclear multiple bond coherence


heteronuclear multiple quantum coherence


high performance liquid chromatography


nuclear magnetic resonance


reactive oxygen species


reverse phase


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Correspondence to Il-Jun Kang.

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Jeon, Y.E., Lee, Y.S., Lim, S.S. et al. Evaluation of the antioxidant activity of the fruiting body of Phellinus linteus using the on-line HPLC-DPPH method. J. Korean Soc. Appl. Biol. Chem. 52, 472–479 (2009).

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