Skip to main content
Applied Biological Chemistry
Download PDF
  • Bioactive Materials
  • Article
  • Published:

Evaluation of the antioxidant activity of the fruiting body of Phellinus linteus using the on-line HPLC-DPPH method

Journal of the Korean Society for Applied Biological Chemistry volume 52, pages 472–479 (2009)Cite this article

Abstract

Phellinus linteus has traditionally been used as a medicinal mushroom in Oriental countries. Bioactive phytochemicals from the fruiting body of P. Linteus were investigated using the on-line HPLC-DPPH system. The system was used to separate 10 antioxidants: protocatechuic acid (1) protocatechualdehyde, (2) caffeic acid (3), ellagic acid (4), hispidin (5), davalliallactone (6), interfungins A (7), hypholomine B (8), inoscavin A (9), and methyldavallialactone (10). Of these, compounds 6 and 7 showed the strongest inhibitory effect against the DPPH radical and superoxide anion radical-scavenging capacity. On comparison with quercetin (IC50, 44.0 μM), the IC50 value of compounds 6 and 7 for DPPH radical-scavenging capacity were 19.6 μM and 18.5 μM. Moreover, the antioxidative capacity of compound 6 at 40mM was 2.95 nmol Trolox equivalents per mg dry wt. These results indicated that hispidin dimers such as hispidin with hispidin or hispolon had a stronger effect on radical-scavenging capacity than hispidin itself.

Abbreviations

DPPH:

2,2-dipheny-1-picrylhydrazyl

ESI:

electospray ionization

HMBC:

heteronuclear multiple bond coherence

HMQC:

heteronuclear multiple quantum coherence

HPLC:

high performance liquid chromatography

NMR:

nuclear magnetic resonance

ROS:

reactive oxygen species

RP:

reverse phase

References

  • Apati P, Szentmihalyi K, Kristo ST, Papp I, Vinkler P, Szoke E, and Kery A (2003) Herbal remedies of Solidago—correlation of phytochemical characteristics and antioxidative properties. J Pharm Biomed Anal 32, 1045–1053.

    Article  CAS  Google Scholar 

  • Bandoniene D and Murkovic M (2002a) The detection of radical scavenging compounds in crude extract of borage (Borago officinalis L.) by using an on-line HPLCDPPH method. J Biochem Bioph Meth 53, 45–49.

    Article  CAS  Google Scholar 

  • Bandoniene D and Murkovic M (2002b) On-line HPLCDPPH screening method for evaluation of radical scavenging phenols extracted from apples (Malus domestica L.). J Agric Food Chem 50, 2482–2487.

    Article  CAS  Google Scholar 

  • Cao G, Sofic E, and Prior RL (1997) Antioxidant and prooxidant behavior of flavonoids: structure-activity relationships. Free Radic Biol Med 22, 749–760.

    Article  CAS  Google Scholar 

  • Chang HY, Ho YL, Sheu MJ, Lin YH, Tseng MC, Wu SH, and Huang GJ (2007) Antioxidant and free radical scavenging capacities of Phellinus merrillii extracts. Bot Stud 48, 407–417.

    CAS  Google Scholar 

  • Chen W, He FY, and Li YQ (2006) The apoptosis effect of hispolon from Phellinus linteus (Berkeley & Curtis) Teng on human epidermoid KB cells. J Ethnopharmacol 105, 280–285.

    Article  CAS  Google Scholar 

  • Cui CB, Tezuka Y, Kikuchi T, Nakano H, Tamaoki T, and Park JH (1990) Constituents of a fern, Davallia mariesii Moore. I. Isolation and structures of davallialactone and a new flavanone glucuronide. Chem Pharm Bull 38, 3218–3225.

    Article  CAS  Google Scholar 

  • Dapkevicius A, van Beek TA, and Niederlander HA (2001) Evaluation and comparison of two improved techniques for the on-line detection of antioxidants in liquid chromatography eluates. J Chromatogr A 912, 73–82.

    Article  CAS  Google Scholar 

  • Evans JL, Goldfine ID, Maddux BA, and Grodsky GM (2002) Oxidative stress and stress-activated signaling pathways: a unifying hypothesis of type 2 diabetes. Endocr Rev 23, 599–622.

    Article  CAS  Google Scholar 

  • Gonindard C, Bergonzi C, Denier C, Sergheraert C, Klaebe A, Chavant L, and Hollande E (1997) Synthetic hispidin, a PKC inhibitor, is more cytotoxic toward cancer cells than normal cells in vitro. Cell Biol Toxicol 13, 141–153.

    Article  CAS  Google Scholar 

  • Halliwell B (1997) Antioxidants and human disease: a general introduction. Nutr Rev 55, S44–49; discussion S49-52.

    Article  Google Scholar 

  • Hwang EI, Kim JR, Jeong TS, Lee S, Rho MC, and Kim SU (2006) Phellinsin A from Phellinus sp. PL3 exhibits antioxidant activities. Planta Med 72, 572–575.

    Article  CAS  Google Scholar 

  • Jung SH, Lee JM, Lee HJ, Kim CY, Lee EH, and Um BH (2007) Aldose reductase and advanced glycation endproducts inhibitory effect of Phyllostachys nigra. Biol Pharm Bull 30, 1569–1572.

    Article  CAS  Google Scholar 

  • Koleva, II, Niederlander HA, and van Been TA (2000) An on-line HPLC method for detection of radical scavenging compounds in complex mixtures. Anal Chem 72, 2323–2328.

    Article  CAS  Google Scholar 

  • Lee IK and Yun BS (2007) Highly oxygenated and unsaturated metabolites providing a diversity of hispidin class antioxidants in the medicinal mushrooms Inonotus and Phellinus. Bioorg Med Chem 15, 3309–3314.

    Article  CAS  Google Scholar 

  • Lee YS, Kang YH, Jung JY, Kang IJ, Han SN, Chung JS, Shin HK, and Lim SS (2008a) Inhibitory constituents of aldose reductase in the fruiting body of Phellinus linteus. Biol Pharm Bull 31, 765–768.

    Article  CAS  Google Scholar 

  • Lee YS, Kang YH, Jung JY, Lee S, Ohuchi K, Shin KH, Kang IJ, Park JH, Shin HK, and Lim SS (2008b) Protein glycation inhibitors from the fruiting body of Phellinus linteus. Biol Pharm Bull 31, 1968–1972.

    Article  CAS  Google Scholar 

  • Min BS, Yun BS, Lee HK, Jung HJ, Jung HA, and Choi JS (2006) Two novel furan derivatives from Phellinus linteus with anti-complement activity. Bioorg Med Chem Lett 16, 3255–3257.

    Article  CAS  Google Scholar 

  • Park IH, Jeon SY, Lee HJ, Kim SI, and Song KS (2004) A beta-secretase (BACE1) inhibitor hispidin from the mycelial cultures of Phellinus linteus. Planta Med 70, 143–146.

    Article  CAS  Google Scholar 

  • Rice-Evans CA, Miller NJ, and Paganga G (1997) Cerebellar agenesis: a case report with clinical and MR imaging findings and a review of the literature. Eur J Neurol 2, 152–159.

    Google Scholar 

  • Sakthivelu G, Akitha Devi MK, Giridhar P, Rajasekaran T, Ravishankar GA, Nikolova MT, Angelov GB, Todorova RM, and Kosturkova GP (2008) Isoflavone composition, phenol content, and antioxidant activity of soybean seeds from India and Bulgaria. J Agric Food Chem 56, 2090–2095.

    Article  CAS  Google Scholar 

  • Velioglu YS, Mazza G, Gao L, and Oomah BD (1998) Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem 46, 4113–4117.

    Article  CAS  Google Scholar 

  • Wang Y, Wang SJ, Mo SY, Li S, Yang YC, and Shi JG (2005) Phelligridimer A, a highly oxygenated and unsaturated 26-membered macrocyclic metabolite with antioxidant activity from the fungus Phellinus igniarius. Org Lett 7, 4733–4736.

    Article  CAS  Google Scholar 

  • Xu B and Chang SK (2009) Total phenolic, phenolic acid, anthocyanin, flavan-3-ol, and flavonol profiles and antioxidant properties of pinto and black beans (Phaseolus vulgaris L.) as affected by thermal processing. J Agric Food Chem 57, 4754–4764.

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Sun-Ju Kim

    Present address: Division of Bioresources Engineering, College of Agriculture and Life Sciences, Chungnam National University, 305-764, Daejeon, Korea

Authors and Affiliations

  1. Department of Food Science and Nutrition, Center for Efficacy Assessment and Development of Functional Foods and Drugs (RIC), Hallym University, 200-702, Chuncheon, Republic of Korea

    Young Eun Jeon, Yeon Sil Lee, Soon Sung Lim & Il-Jun Kang

  2. Institute of Natural Medicine, College of Medicine, Hallym University, 200-702, Chuncheon, Republic of Korea

    Soon Sung Lim & Sun-Ju Kim

  3. Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute, 210-340, Gangneung, Republic of Korea

    Sang Hoon Jung

  4. Department of Wood Science and Engineering, Kangwon National University, 200-701, Chuncheon, Republic of Korea

    Young-Soo Bae

  5. Division of Forest Resources, Kangwon National University, 200-701, Chuncheon, Republic of Korea

    Jae-Seon Yi

Authors
  1. Young Eun Jeon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yeon Sil Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Soon Sung Lim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sun-Ju Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sang Hoon Jung
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Young-Soo Bae
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jae-Seon Yi
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Il-Jun Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Il-Jun Kang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jeon, Y.E., Lee, Y.S., Lim, S.S. et al. Evaluation of the antioxidant activity of the fruiting body of Phellinus linteus using the on-line HPLC-DPPH method. J. Korean Soc. Appl. Biol. Chem. 52, 472–479 (2009). https://doi.org/10.3839/jksabc.2009.081

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.3839/jksabc.2009.081

Key words