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The anti-melanogenic effects of Petalonia binghamiae extracts in α-melanocyte stimulating hormone-induced B16/F10 murine melanoma cells

Journal of the Korean Society for Applied Biological Chemistry volume 52, pages 564–567 (2009)Cite this article

Abstract

Petalonia binghamiae extracts (PBE) suppressed melanin synthesis in a dose-dependent manner in α-melanocyte stimulating hormone (α-MSH)-treated B16/F10 murine melanoma cells. Specifically, the cell tyrosinase activity and melanin content were inhibited by 72% and 48%, respectively, in response to treatment with 100 μg/mL of PBE. The results of western blot analysis suggest that PBE induced the inhibition of tyrosinase and TRP-1 protein expression through suppression of α-MSH induced p38 and ERK activation.

Abbreviations

ERK:

extracellular signal-regulated kinase

α-MSH:

α-melanocyte stimulating hormone

MTT:

3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide

PBE:

Petalonia Binghamiae extracts

TRP:

tyrosinase-related protein

References

  • Briganti S, Camera E, and Picardo M (2003) Chemical and instrumental approaches to treat hyperpigmentation. Pigment Cell Res 16, 101–110.

    Article  Google Scholar 

  • Corre S, Primot A, Sviderskaya E, Bennett DC, Vaulont S, Goding CR, and Galibert MD (2004) UV-induced expression of key component of the tanning process, the POMC and MC1R genes, is dependent on the p-38-activated upstream stimulating factor-1 (USF-1). J Biol Chem 279, 51226–51233.

    Article  CAS  Google Scholar 

  • Gilchrest BA, Park HY, Eller MS, and Yaar M (1996) Mechanisms of ultraviolet light-induced pigmentation. Photochem Photobiol 63, 1–10.

    Article  CAS  Google Scholar 

  • Goding CR (2006) Mitf from neural crest to melanoma: signal transduction and transcription in the melanocyte lineage. Genes Dev 14, 1712–1728.

    Google Scholar 

  • Hearing VJ and Tsukamoto K (1991) Enzymatic control of pigmentation in mammals. FASEB J 5, 2902–2909.

    CAS  Google Scholar 

  • Hill HZ, Li W, Xin P, and Michell DL (1997) Melanin: a two edged sword? Pigment Cell Res 10, 158–161.

    Article  CAS  Google Scholar 

  • Imokawa G, Kobayashi T, Miyagishi M, Higashi K, and Yada Y (1997) The role of endrothelin-1 in epidermal hyperpigmentation and signaling mechanisms of mitogenesis and melanogenesis. Pigment Cell Res 10, 218–228.

    Article  CAS  Google Scholar 

  • Kang JR, Lee MK, and Kang SM (2008a) Anti-oxidant property and tyrosinase inhibition activity of various extracts from plants in Compositae plants. J Korean Soc Appl Biol Chem 51, 321–328.

    Article  CAS  Google Scholar 

  • Kang SI, Jin YJ, Ko HC, Choi SY, Hwang JH, Whang I, Kim MH, Shin HS, Jeong HB, and Kim SJ (2008b) Petalonia improves glucose homeostasis in streptozotocin-induced diabetic mice. Biochem Biophys Res Commun 373, 265–269.

    Article  CAS  Google Scholar 

  • Kobayashi T, Imokawa G, Bennett DC, and Hearing VJ (1998) Tyrosinase stabilization by Tyrp1 (the brown locus protein). J Biol Chem 273, 31801–31805.

    Article  CAS  Google Scholar 

  • Kuda T, Hishi T, and Maekawa S (2006) Anti-oxidant properties of dried product of ‘habanori’, an edible brown alga, Petalonia binhamiae (J. Agaradh) Vinogrdova. Food Chem 98, 545–550.

    Article  CAS  Google Scholar 

  • Martinez-Liarte JH, Solano F, Garcia-Borron JC, Jara JR, and Lozano JA (1992) α-MSH and other melanogenic activators mediate opposite effects on tyrosinase and dopachrome tautomerase in B16/F10 mouse melanoma cells. J Invest Dermatol 99, 435–439.

    Article  CAS  Google Scholar 

  • Mizushina Y, Sugiyama Y, Yoshida H, Hanashima S, Yamzaki T, Kamisuki S, Ohta K, Takemura M, Yamaguchi T, Matsukage A, Yoshida S, Saneyoshi M, Sugawara F, and Sakagauchi K (2001) Galactosyl-diacylglycerol, a mammalian DNA polymerase α-specific inhibitor from a sea alga, Petalonia binhamiae. Biol Pharm Bull 24, 982–987.

    Article  CAS  Google Scholar 

  • Mori K, Ooi T, Hiraoka M, Oka N, Hamada H, Tamuara M, and Kusumi T (2004) Fucoxanthin and its metabolites in edible brown algae cultivated in deep seawater. Mar Drugs 2, 63–72.

    Article  CAS  Google Scholar 

  • Thody AJ and Graham A (1998) Does alpha-MSH have a role in regulating skin pigmentation in humans? Pigment Cell Res 11, 265–274.

    Article  CAS  Google Scholar 

  • Tsukamoto K, Jackson IJ, Urabe K, Montague PM, and Hearing VJ (1992) A second tyrosinase-related protein, TRP-2, is a melanogenic enzyme termed DOPAchrome tautomerase. EMBO J 11, 519–526.

    CAS  Google Scholar 

  • Yanase H, Ando H, Horikawa M, Watanabe M, Mori T, and Matsuda N (2001) Possible involvement of ERK 1/2 in UVA-induced melanogenesis in cultured normal human epidermal melanocytes. Pigment Cell Res 14, 103–109.

    Article  CAS  Google Scholar 

  • Yoon HS and Kim JK (2007) The inhibitory effect of Acanthopeltis japonica on melanogenesis. J Soc Cosmet Sci Korea 33, 87–92.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Subtropical Horticulture Research Institute, 690-756, Jeju, Republic of Korea

    Hoon Seok Yoon & In-Jung Kim

  2. Faculty of Biotechnology, College of Applied Life Sciences, 690-756, Jeju, Republic of Korea

    Won Beom Koh & In-Jung Kim

  3. Research Institute for Subtropical Agriculture and Biotechnology, 690-756, Jeju, Republic of Korea

    In-Jung Kim

  4. Biotechnology Regional Innovation Center, Jeju National University, 690-756, Jeju, Republic of Korea

    You-Sung Oh

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  1. Hoon Seok Yoon
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  2. Won Beom Koh
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  3. You-Sung Oh
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  4. In-Jung Kim
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Correspondence to In-Jung Kim.

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Yoon, H.S., Koh, W.B., Oh, YS. et al. The anti-melanogenic effects of Petalonia binghamiae extracts in α-melanocyte stimulating hormone-induced B16/F10 murine melanoma cells. J. Korean Soc. Appl. Biol. Chem. 52, 564–567 (2009). https://doi.org/10.3839/jksabc.2009.095

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  • DOI: https://doi.org/10.3839/jksabc.2009.095

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