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Hemolysin Gene Expression in the hns Knockout Mutant of Klebsiella pneumoniae UN Strain

Journal of the Korean Society for Applied Biological Chemistry volume 53, pages 401–406 (2010)Cite this article

Abstract

Hemolysin proteins are exotoxins produced by bacteria that can cause lysis of red blood cells in vitro. Hemolysin E is a pore-forming hemolysin, one of three types of hemolysin. Generally, hemolysin E is not expressed under standard growth conditions such as growth in LB medium at 37°C. However, in E. coli, when the hns gene, which encodes H-NS, a global regulatory DNA binding protein, is knocked out, hemolysin E gene is expressed. Because hns genes in E. coli and K. pneumoniae are similar, K. pneumoniae UN strain hns gene was knocked out in this research. The hns knockout mutant of K. pneumoniae UN is found to produce a 19 kDa hemolysin protein and shows hemolytic activity.

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Authors and Affiliations

  1. Department of Biotechnology, Chung-Ang University, 456-756, Anseong, Republic of Korea

    Jang Suk Han, In-Young Jang, Hyung-Seok Ryu & Woo-Yeon Kim

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  1. Jang Suk Han
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  2. In-Young Jang
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  3. Hyung-Seok Ryu
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  4. Woo-Yeon Kim
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Correspondence to Woo-Yeon Kim.

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Han, J.S., Jang, IY., Ryu, HS. et al. Hemolysin Gene Expression in the hns Knockout Mutant of Klebsiella pneumoniae UN Strain. J. Korean Soc. Appl. Biol. Chem. 53, 401–406 (2010). https://doi.org/10.3839/jksabc.2010.062

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  • DOI: https://doi.org/10.3839/jksabc.2010.062

Key words

  • hemolysin
  • H-NS
  • Klebsiella pneumoniae UN