- Bioactive Materials
- Article
- Published:
Effect of Hydroxycinnamic Acid Derivatives from Corn Bran on Melanogenic Protein Expression
Journal of the Korean Society for Applied Biological Chemistry volume 53, pages 422–426 (2010)
Abstract
A recent study has demonstrated that polyamine conjugates from corn (Zea may L.) bran and related hydroxycinnamic acids have antioxidant and antimelanogenic activities. To verify the ability of hydroxycinnamic acid derivatives (HCAD) from corn bran to reduce skin pigmentation as cosmetic active ingredients, we assessed the effect on melanogenic proteins expression in murine B16 melanoma cells and on skin pigmentation levels in clinical trials. We investigated the effect of HCAD on melanogenic protein expression using Western blot analysis. Furthermore, we assessed the effects of a cream containing 0.1% by weight corn bran extract (CBE) with HCAD such as N,N′-dicoumaroylputrescine, N-p-coumaroyl-N′-feruloylputrescine and N,N′-diferuloylputrescine in clinical trials. HCAD reduced the forskolin-induced increase in melanin content of cultured melanoma cells. To elucidate the effect of HCAD on melanogenesis, we performed a Western blot analysis of melanogenic proteins, such as tyrosinase, tyrosinase-related protein (TRP)-1, TRP-2 and microphthalmia-associated transcription factor (MITF). HCAD reduced levels of tyrosinase and MITF, however, it did not change TRP-1 and TRP-2 levels. The 0.1% CBE cream significantly reduced skin pigmentation with no irritation. The present study indicates that HCAD have good potential to be useful antimelanogenic agents.
References
Choi SW, Lee SK, Kim EO, Oh JH, Yoon K-S, Parris N, Hicks KB, and Moreau RA (2007) Antioxidant and antimelanogenic activities of polyamine conjugates from corn bran and related hydroxycinnamic acids. J Agric Food Chem 55, 3920–3925.
Friedmann PS and Gilchrest BA (1987) Ultraviolet radiation directly induces pigment production by cultured human melanocytes. J Cell Physiol 133, 88–94.
Halaban R, Pomerantz SH, Marshall S, and Lerner AB (1984) Tyrosinase activity and abundance in Cloudman melanoma cells. Arch Biochem Biophys 230, 383–387.
Hunt G, Todd C, Creswell JE, and Thody AJ (1994) Alpha-melanocyte stimulating hormone and its analogue Nle 4DPhe 7 alpha-MSH affect morphology, tyrosinase activity and melanogenesis in cultured human melanocytes. J Cell Sci 107, 205–211.
Iwai K, Kishimoto N, Kakino Y, Mochida K, and Fujita T (2004) In vitro antioxidative effects and tyrosinase inhibitory activities of seven hydroxycinnamoyl derivatives in green coffee beans. J Agric Food Chem 52, 4893–4898.
Jimenez-Cervantes C, Garcia-Borron JC, Valverde P, Solano F, and Lozano JA (1993) Tyrosinase isoenzymes in mammalian melanocytes. 1. Biochemical characterization of two melanosomal tyrosinases from B16 mouse melanoma. Eur J Biochem 217, 549–556.
Kim MJ, Im KR, Yoon K-S, and Choi SW (2009) The inhibitory effect of hydroxycinnamic acid derivatives from corn (Zea may L.) bran on melanogenesis. J Soc Cosmet Scientists Korea 35, 143–149.
Lee J, Jung E, Park J, Jung K, Park E, Kim J, Hong S, Park J, Park S, Lee S, and Park D (2005) Glycyrrhizin induces melanogenesis by elevating a cAMP level in B16 melanoma cells. J Invest Dermatol 124, 405–411.
Lee J, Jung K, Kim YS, and Park D (2007) Diosgenin inhibits melanogenesis through the activation of phosphatidylinositol-3-kinase pathway (PI3K) signaling. Life Sci 81, 249–254.
Natella F, Nardini M, Di Felice M, and Scaccini C (1999) Benzoic and cinnamic acid derivatives as antioxidants: Structure-activity relation. J Agric Food Chem 47, 1453–1459.
Oka M, Ichihashi M, and Chakraborty AK (1996) Enhanced expression of protein kinase C subspecies in melanogenic compartments in B16 melanoma cells by UVB or MSH. J Invest Dermatol 106, 377–378.
Perez-Alvarez V, Bobadilla RA, and Muriel P (2001) Structure hepatoprotective activity relationship of 3,4-dihydroxy-cinnamic acid (caffeic acid) derivatives. J Appl Toxicol 21, 527–531.
Piérard GE (1998) EEMCO guidance for the assessment of skin colour. J Eur Acad Dermatol Venereol 10, 1–11.
Ramos-Nino ME, Clifford MN, and Adams MR (1996) Quantitative structure activity relationship for the effects of benzoic acids, cinnamic acids and benzaldehydes on Listeria monocytogenes. J Appl Bacteriol 80, 303–310.
Steingrimsson E, Copeland NG, and Jenkins NA (2004) Melanocytes and the microphthalmia transcription factor network. Annu Rev Genet 38, 365–411.
Wong G and Pawelek J (1975) Melanocyte-stimulating hormone promotes activation of pre-existing tyrosinase molecules in Cloudman S91 melanoma cells. Nature 255, 644–646.
Yamada J and Tomita Y (1996) Antimutagenic activity of caffeic acid and related compounds. Biosci Biotechnol Biochem 60, 328–329.
Zhang LP and Ji ZZ (1992) Synthesis, antiinflammatory and anticancer activity of cinnamic acids, their derivatives and analogues. Yao Xue Xue Bao (Acta Pharmaceutica Sinica) 27, 817–823.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kim, M.J., Kim, S.M., Im, K.R. et al. Effect of Hydroxycinnamic Acid Derivatives from Corn Bran on Melanogenic Protein Expression. J. Korean Soc. Appl. Biol. Chem. 53, 422–426 (2010). https://doi.org/10.3839/jksabc.2010.065
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.3839/jksabc.2010.065