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Bacteriophages of Pseudomonas tolaasii for the biological control of brown blotch disease

Journal of the Korean Society for Applied Biological Chemistry volume 54, pages 99–104 (2011)Cite this article

Abstract

Pseudomonas tolaasii causes brown blotch disease in cultivated mushrooms by producing tolaasin, a peptide toxin, which forms pores on the membrane and disrupts the cellular and fruiting body structures of mushrooms. For the biological control of this mushroom disease, virulent bacteriophages of P. tolaasii were isolated from the sewage of Cheongju, Korea. Twenty-one phages were isolated from four different locations, and their toxicities to host bacteria were measured by inspecting the turbidity and size of their plaques. They were divided into three categories on the basis of their toxicities to host bacteria. In order to test if these phages can be used for the biological control of mushroom diseases, a pitting test was performed. The surfaces of mushroom caps were inoculated with both pathogenic bacteria and their phages. Phage toxicity was analyzed by measuring the size of the blotches that formed on the surface of mushrooms, because these sizes are representative of the amount of tolaasin peptide produced by pathogenic bacteria in the presence of bacteriophages. The formation of blotches was completely blocked by co-incubated phages. These results show that phages can sterilize pathogenic bacteria in mushroom tissues as well as be useful for the biological control of brown blotch disease. The optimum conditions for the bactericidal activity of the phages were also determined.

References

  • Adams MH (1959) In Bacteriophages, Interscience Publishers, New York, NY, USA.

    Google Scholar 

  • Chakrabarti AK, Ghosh AN, Balakrish Nair G, Niyogi SK, Bhattacharya SK, and Sarkar BL (2000) Development and evaluation of a phage typing scheme for Vibrio cholerae O139. J Clin Microbiol 38, 44–49.

    CAS  Google Scholar 

  • Chanishvili N, Chanishvili T, Tediashvili M, and Barrow PA (2001) Phages and their application against drug-resistant bacteria. J Chem Technol Biotechnol 76, 689–699.

    Article  CAS  Google Scholar 

  • Chibani-Chennoufi S, Sidoti J, Bruttin A, Kutter E, Sarker S, and Brussow H (2004) In vitro and in vivo bacteriolytic activities of Escherichia coli phages: implications for phage therapy. Antimicrob Agents Chemother 48, 2558–2569.

    Article  CAS  Google Scholar 

  • Cooper TF and Heinemann JA (2000) Transfer of conjugative plasmids and bacteriophage ë occurs in the presence of antibiotics that prevent de novo gene expression. Plasmid 43, 171–175.

    Article  CAS  Google Scholar 

  • Gandy DG (1968) A technique for screening bacteria causing brown blotch of cultivated mushrooms. In Annual Report of the Glasshouse Crops Research Institute for 1967, pp. 150–154, Glasshouse Crops Research Institute, Littlehampton, West Sussex, UK.

    Google Scholar 

  • Geels FP (1995) Pseudomonas tolaasii control by kasugamycin cultivated mushrooms (Agaricus bisporus). J Appl Bacteriol 79, 38–42.

    Article  CAS  Google Scholar 

  • Geels FP, van Griensven LJLD, and Rutjens AJ (1991) Chlorine dioxide and the control of bacterial blotch on mushrooms, caused by Pseudomonas tolaasii. In Science and Cultivation of Edible Fungi, Maher MJ (ed.), 1, pp.437–442, Balkema Pub., Brookfield, VT, USA.

    Google Scholar 

  • Greer GG (2005) Bacteriophage control of foodborne bacteria. J Food Prot 68, 1102–1111.

    Google Scholar 

  • Guillaumes J, Houdeau G, Germain R, and Oliver JM (1998) Amelioration de la lutte biologique contre Pseudomonas tolaasii utilisation de bacteriophages. Bull OEPP/EPPO 18, 77–82.

    Article  Google Scholar 

  • Hadas H, Einav M, Fishov I, and Zaritsky A (1997) Bacteriophage T4 development depends on the physiology of its host E. coli. Microbiology 143, 179–185.

    Article  CAS  Google Scholar 

  • Hutchison MI and Johnstone K (1993) Evidence for the involvement of the surface active properties of the extracellular toxin tolaasin in the manifestation of brown blotch disease symptoms by Pseudomonas tolaasii on Agaricus bisporus. Physiol Mol Plant Pathol 42, 273–384.

    Article  Google Scholar 

  • Kim JW, Kim KH, and Kang HJ (1994) Studies on the pathogenic Pseudomonas causing bacterial disease of cultivated mushroom in Korea: 1. On the causal organisms of the rots of Agaricus bisporus, Pleurotus ostreatus, and Lentinus edodes. Kor J Plant Pathol 10, 197–210.

    Google Scholar 

  • Kim ST, Choi TK, and Kim YK (2007) pH-dependent cytotoxicity of a peptide toxin, tolaasin. J Kor Soc Appl Biol Chem 50, 257–261.

    CAS  Google Scholar 

  • Lee HI, Lee SD, Park KS, Kim YK, and Cha JS (1997) Pathogenicity of bacterial isolates from brown blotch diseased oyster mushrooms in Chungcheungbuk-do. J Agr Sci Chungbuk Nat’l Univ 14, 121–132.

    Google Scholar 

  • Matsuzaki S, Rashel M, Uchiyama J, Sakurai S, Ujihara T, Kuroda M, and Imai S (2005) Bacteriophage therapy: A revitalized therapy against bacterial infectious disease. J Infect Chemother 11, 211–219.

    Article  Google Scholar 

  • Moebus K (1996) Marine bacteriophage reproduction under nutrient-limited growth of host bacteria. II. Investigations with phage-host system (H3:H3/1). Mar Ecol Prog Ser 144, 13–22.

    Article  Google Scholar 

  • Munsch P, Oliver JM, and Houdeau G (1991) Experimental control of bacterial blotch by bacteriophages. In Science and Cultivation of Edible Fungi, Maher MJ (ed.), 1, pp. 389–396, Balkema Pub, Rotterdam, Netherlands.

    Google Scholar 

  • Peng JT (1986) Resistance to disease in Agaricus bisporus (Lange) Imbach. Ph.D. Thesis, University of Leeds, Leeds, UK.

    Google Scholar 

  • Sambrook J and Russell DW (2001) In Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, New York, NY, USA.

    Google Scholar 

  • Sillankorva S, Oliveira R, Vieira MJ, Sutherland I, and Azeredo J (2004) Pseudomonas fluorescens infection by bacteriophage Ï•S1: the influence of temperature, host growth phase and media. FEMS Microbiol Lett 241, 13–20.

    Article  CAS  Google Scholar 

  • Soler-Rivas C, Arpin N, Olivier JM, and Wichers HJ (1999) WLIP, a lipodepsipetide of Pseudomonas ‘reactans’, as inhibitor of the symptoms of the brown blotch disease of Agaricus bisporus. J Appl Microbiol 86, 635–641.

    Article  CAS  Google Scholar 

  • Tolaas AG (1915) A bacterial disease of cultivated mushrooms. Phytopathol 5, 51–54.

    Google Scholar 

  • Tsuneda A, Suyama K, Murakami S, and Ohira I (1995) Occurrence of Pseudomonas tolaasii on fruiting bodies of Lentinula edodes formed on Quercus logs. Mycoscience 36, 283–288.

    Article  Google Scholar 

  • Wong WC and Preece TF (1985) Pseudomonas tolaasii in cultivated mushroom (Agaricus bisporus) crops effect of sodium hydrochloride on the bacterium and on blotch disease severity. J Appl Bacteriol 58, 259–267.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Environmental and Biological Chemistry, Chungbuk National University, 361-763, Cheongju, Korea

    Min-Hee Kim, Seong-Wan Park & Young-Kee Kim

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  1. Min-Hee Kim
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  2. Seong-Wan Park
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Correspondence to Young-Kee Kim.

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Kim, MH., Park, SW. & Kim, YK. Bacteriophages of Pseudomonas tolaasii for the biological control of brown blotch disease. J. Korean Soc. Appl. Biol. Chem. 54, 99–104 (2011). https://doi.org/10.3839/jksabc.2011.014

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