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Screening and compound isolation from natural plants for anti-allergic activity

Journal of the Korean Society for Applied Biological Chemistry volume 54, pages 367–375 (2011)Cite this article

Abstract

Drug discovery for the treatment of allergic disease is an important field in human health. Natural plants and medicinal herbs used commercially in Korea were screened for degranulation inhibitory activities of 164 plant species in the RBL-2H3 cell line, and their activities were investigated using the β-hexosaminidase release assay; 34 species were active. Lactuca indica var. laciniata had the highest degranulation inhibitory activity, and Plantago asiatica, Cimicifuga heracleifolia, Poncirus trifoliate, Lycopus ramosissimus, Quercus mongolica, Anemarrhena asphodeloides, Eugenia caryophyllata, and Curcuma longa exhibited degranulation inhibitory capacity higher than 50% at 125 μg/mL. One compound was isolated by activity-guided fractionation and isolation from methanol (MeOH) extract of A. asphodeloides, which has not yet been identified as constituent for anti-allergic and atopic effects. The structure of isolated compound was elucidated on the basis of NMR and Mass spectrometric data as nyasol, which was highly effective at very low dose (70.98±1.57% at 50 μg/mL). These results demonstrate that natural products and contained compounds may be useful in the therapy of allergic diseases, such as atopic dermatitis and asthma.

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Authors and Affiliations

  1. Department of Natural Medicine Resources, Semyung University, 390-711, Jecheon, Republic of Korea

    Jong Phil Bak, Jong Hyuk Park, Yoon Jung Yang & Se Chan Kang

  2. Department of Biotechnology, College of Biomedical & Health Sciences, Konkuk University, 380-701, Choong-Ju, Republic of Korea

    Jong Bo Kim

  3. Department of Chemistry, University of Ulsan, 680-749, Ulsan, Republic of Korea

    In Su Kim

  4. DanjoungBio Ltd., Co., 220-842, Wonju, Republic of Korea

    Eui Su Choung

Authors
  1. Jong Phil Bak
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  2. Jong Bo Kim
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  3. Jong Hyuk Park
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  4. Yoon Jung Yang
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  5. In Su Kim
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  6. Eui Su Choung
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  7. Se Chan Kang
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Corresponding author

Correspondence to Se Chan Kang.

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J. P. Bak contributed equally.

J. B. Kim contributed equally.

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Bak, J.P., Kim, J.B., Park, J.H. et al. Screening and compound isolation from natural plants for anti-allergic activity. J. Korean Soc. Appl. Biol. Chem. 54, 367–375 (2011). https://doi.org/10.3839/jksabc.2011.058

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  • DOI: https://doi.org/10.3839/jksabc.2011.058

Key words

  • allergy
  • degranulation
  • medicinal herbs
  • RBL-2H3