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Inhibition of browning on fresh apple juices by natural phytochemicals from Rumex crispus L. seed

Abstract

Efficacy of phytochemicals of Rumex crispus L. seeds as an anti-browning agent was examined. Anti-browning candidates were screened using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Antioxidant activities of DPPH radical scavengers increased with increasing amount of the extracts. Butanol (BE) and ethyl acetate extracts (EE) displayed significantly higher DPPH radical-scavenging activity as compared to that of ascorbic acid. Anti-browning activity was evaluated by monitoring the change of L *, a *, and b * values as well as total color differences (E). BE and EE effectively inhibited browning in apple juice at a concentration below 0.3 mg mL−1, whereas other extracts did not show anti-browning effects in apple juice. R. crispus L. seed extracts could be of good resources as anti-browning agents. Results indicate possible contribution to the development of new and natural anti-browning agents with potential applications to reduce oxidative damage.

References

  • Brand-Williams W, Cuvelier ME, and Berset C (1995) Use of a free radical method to evaluate antioxidant activity. Lebensmittel Wissenschaft und Technologie 28, 25–30.

    CAS  Google Scholar 

  • Choi GJ, Lee SW, Jang KS, Kim JS, Cho KY, and Kim JC (2005) Effects of chrysophanol, parietin, and nepodin of Rumex crispus on barley and cucumber powdery mildews. Crop Prot 23, 1215–1221.

    Google Scholar 

  • Gacche RN, Warangkar SC, and Ghole VS (2004) Glutathione and cinnamic acid: natural dietary components used in preventing the process of browning by inhibition of polyphenol oxidase in apple juice. J Enzyme Inhib Med Chem 19, 175–179.

    CAS  Google Scholar 

  • Gui F, Wu J, Chen F, Liao X, Hu X, Zhang Z, and Wang Z (2006) Change of polyphenol oxidase activity, color, and browning degree during storage of cloudy apple juice treated by supercritical carbon dioxide. Eur Food Res Technol 223, 427–432.

    CAS  Google Scholar 

  • Jeon M and Zhao Y (2005) Honey in combination with vacuum impregnation to prevent enzymatic browning of fresh-cut apple. Int J Food Sci Nutr 56, 165–176.

    CAS  Google Scholar 

  • Ko FN, Cheng ZJ, Lin CN, and Teng CH (1998) Scavenger and antioxidant properties of prenylflavones isolated from Artocarpus heterophyllus. Free Radic Biol Med 25, 160–168.

    CAS  Google Scholar 

  • Krapfenbauer G, Kinner M, Gossinger M, Schonlechner R, and Berqhofer E (2006) Effect of thermal treatment on the quality of cloudy apple juice. J Agric Food Chem 54, 5453–5460.

    CAS  Google Scholar 

  • Kubo I, Kinst-Hori I, Kubo Y, Yamagiwa Y, Kamikawa T, and Haraguchi H (2000) Molecular design of anti-browning agents. J Agric Food Chem 48, 1393–1399.

    CAS  Google Scholar 

  • Leston G (2000) (Polyhydroxy) Benzenes, In Kirk-Othmer Encyclopedia of Chemical Technology, John Willey & Sons Inc, West Sussex, England.

    Google Scholar 

  • Li H, Cheng KW, Cho CH, He Z, and Wang M (2007) Oxyresveratrol as an antibrowning agent for cloudy apple juices and fresh-cut apples. J Agric Food Chem 55, 2604–2610.

    CAS  Google Scholar 

  • Luo Y and Barbosa-Canovas GV (1997) Enzymic browning and its inhibition in new apple cultivars slices using 4- hexylresorcinol in combination with ascorbic acid. Int J Food Sci Technol 3, 195–201.

    CAS  Google Scholar 

  • Mchugh TH and Senesi E (2000) Apple wraps: A novel method to improve the quality and extend the shelf life of fresh-cut apples. J Food Sci 65, 480–485.

    CAS  Google Scholar 

  • Molyneux P (2004) The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol 26, 211–219.

    CAS  Google Scholar 

  • Nerya O, Ben-Arie R, Luzzatto T, Musa R, Khativ S, and Vaya J (2006) Prevention of Agaricus bisporus postharvest browning with tyrosinase inhibitors. Postharvest Biol Technol 39, 272–277.

    CAS  Google Scholar 

  • Oms-Oliu G, Aguilo-Aguayo I, and Martin-Belloso O (2006) Inhibition of browning on fresh-cut pear wedges by natural compounds. J Food Sci 71, S216–S224.

    CAS  Google Scholar 

  • Osawa T and Namiki M (1981) A novel type of antioxidant isolated from leaf wax of eucalyptus leaves. Agric Biol Chem 45, 735–739.

    CAS  Google Scholar 

  • Prestamo G and Arroyo G (1999) Protective effect of ascorbic acid against the browning developed in apple fruit treated with high hydrostatic pressure. J Agric Food Chem 47, 3541–3545.

    CAS  Google Scholar 

  • Rojas-Grau MA, Sobrino-Lopez A, Tapia Ma S, and Martin- Belloso O (2006) Browning inhibition in fresh-cut ’fuji’ apple slices by natural anti-browning agents. J Food Sci 71, S59–S65.

    CAS  Google Scholar 

  • Suh HJ, Lee KS, Kim SR, Shin MH, Park SG, and Park S (2011) Determination of singlet oxygen quenching and protection of biological systems by various extracts from seed of Rumex crispus L. J Photochem Photobiol B 102, 102–107.

    CAS  Google Scholar 

  • Yildirim A, Mavi A, and Kara AA (2001) Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts. J Agric Food Chem 49, 4083–4089

    CAS  Google Scholar 

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Suh, HJ., Park, S. & Park, S. Inhibition of browning on fresh apple juices by natural phytochemicals from Rumex crispus L. seed. J Korean Soc Appl Biol Chem 54, 524–530 (2011). https://doi.org/10.3839/jksabc.2011.080

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  • DOI: https://doi.org/10.3839/jksabc.2011.080

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