Fig. 3

MT binding of tau in HCT116 cell line protein from HCT 116 and SH-SH5Y cells, E. coli tau and bovine brain MAPs were incubated with microtubules as illustrated in materials and methods. The same proportions of supernatant (S) and pellet (P) fractions of tau were immunoblotted with anti-tau mAb. Control lanes of MT only (lanes 9–10) were run to confirm that MT does not have any endogenous tau