Skip to main content

Chemical fingerprint analysis of fermented Morinda citrifolia L. (Noni) juice by UHPLC Q-TOF/MS combined with chemometric analysis

Abstract

Morinda citrifolia L. (Noni) has been widely used in traditional medicine in tropical zones and has become increasingly popular globally owing to its health benefits. Most noni fruits are consumed as juice, which is traditionally produced by the natural fermentation of noni fruits. In this study, the metabolic profiles of noni fruit juice (NJ1) and fermented noni fruit juices (NJ2 and NJ3) was compared. A total of 74, 83, and 91 compounds including anthraquinones, coumarins, flavonoids, phenolic acids, phenolics, terpenoids, and miscellaneous (acids, carbohydrates, vitamins, fatty acids, etc.) were tentatively identified from NJ1, NJ2, and NJ3 in both positive and negative electrospray ionization modes. The phenolic compound composition differed significantly between noni juice and fermented noni juice. The results of the unsupervised principal component analysis and hierarchical clustering analysis showed that the non-fermented juice group clustered with the fermented juice groups. Asperulosidic acid, isoasperulosidic acid, and rutin levels were higher in the NJ1 group than those in the NJ2 group. Deacetylasperulosidic acid and monotropein contents in NJ2 were higher than those in NJ1. Similarly, NJ1 had higher asperulosidic acid and isoasperulosidic acid than those in NJ3. The findings from this study have the potential to enhance the quality of fermented noni juice.

Introduction

Noni (Morinda citrifolia L) is widely cultivated in tropical and subtropical regions such as Australia, Polynesia, Hawaii, and other Pacific islands. The fruits and leaves have been used in traditional folk medicine for the treatment of several diseases, such as high blood pressure, inflammation, and diabetes [1]. Modern scientific research has shown that noni fruits possess antioxidant, anti-inflammatory, liver-protective, and immunomodulatory effects [2,3,4]. This fruit contains various bioactive compounds, such as flavonoids, lignans, iridoids, coumarins, anthraquinones, polysaccharides, terpenoids, sterols, fatty acids, organic acids, vitamins, and minerals [2, 5]. Phenolic compounds play a key role in the therapeutic properties of plants; for example, rutin, β-sitosterol, asperuloside, and ursolic acid are important biologically active compounds present in noni [6]. Flavonoids isolated from noni fruits, such as kaempferol, quercetin, catechin, and epicatechin have shown antidepressant and antioxidant activities [7]. Previous studies have shown that fermentation can significantly affect the composition and bioactivity of phenolic compounds. For example, the phenolic composition of Dendrobium candidum substantially changes after fermentation, and the contents of syringic acid, 4-hydroxybenzoic acid, and p-hydroxycinnamic acid increase significantly [8]. Traditionally, noni juice is produced by fermenting noni fruits in sealed jars or barrels for approximately 2 months or longer and then recovering the juice through drip extraction and/or mechanical pressure. Deng et al. reported that the phytochemical fingerprints of 13 commercial noni-fermented juices on the global market and found that they all contained scopolamine, rutin, and quercetin [9].

Fermentation is a natural process that converts sugars into products that are useful to humans using several microorganisms [10]. In addition, fermentation can lead to increased chemical changes in organic substances through the action of enzymes [11]. Lactic acid bacteria (LAB) are used in dairy and non-dairy foods such as yogurt, tea, and fruits. Recently, the demand for non-dairy probiotic products has increased because of an increase in their immune function [12]. An increasing number of investigators are focusing on the biotransformation of phytochemical substances in vegetable and fruit juices using LAB to produce functional beverages [13]. Vegetables and fruits are enriched in phenolics, organic acids, and sugars, which can be metabolized by LAB strains and therefore improve the sensory, nutritional, and functional qualities, as well as extend the shelf life of fermented products. Currently, the noni juice market is growing continuously because of its potential biological activities. Moreover, there are attempts to commercialize noni fruit through various processing methods such as fermentation and drying, and among them, fermented noni fruit juice stands out as the most popular in the market [14]. However, there is very little information on the phytochemical components of fermented noni juice in the modern scientific literature. Therefore, the objective of this study was to explore an extensive investigation of the phytochemical composition of noni juice and fermented noni juice through the application of untargeted metabolomics.

Materials and methods

Plant material

The samples (organic noni fruit juice, NJ1; fermented organic noni fruit juice I, NJ2; and fermented organic noni fruit juice II, NJ3) were provided by the R&D Center of NSTBio Co., Ltd. (Incheon, Korea) and Atomy Orot Co. (Gongju, Chungnam, Korea). Organic noni fruit, imported from Indonesia, was juiced and used as the sample (NJ1: pH 4.5, Brix 8.5). NJ1 was stored frozen at -80 °C until used in the experiment. Organic noni fruits (10 kg) were fermented with 150 mL of a mixture of probiotics (Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus casei, Lactobacillus fermentum, Lactobacillus paracasei, Lactobacillus reuteri, Lactococcus lactis subsp. Lactis) at 37 °C for 45 d (NJ2: pH 3.9, Brix 8.0). Initially, the inoculum size was 2.4 × 107 CFU/g. NJ3 (pH 3.9, Brix 15.0) was prepared by adding organic coconut blossom sugars, calamansi juice, and organic maltodextrin to NJ2.

Chemicals and reagents

High-performance liquid chromatography (HPLC)-grade acetonitrile (ACN) was obtained from Fisher Scientific (Seoul, Korea), and formic acid was purchased from Merck (Darmstadt, Germany). HPLC-grade methanol and isopropanol (IPA) were supplied by Honeywell Burdick & Jackson (Honeywell Burdick & Jackson, Muskegon, MI, USA) and deionized water was obtained using a Milli-Q water purification system (Millipore Ltd., Bedford, MA, USA). The standards (purity ≥ 95%) of catechin, deacetylasperulosidic acid, epicatechin, gallic acid, hesperidin, isoquercitrin, naringin, nicotinamide, nicotinic acid, p-coumaric acid, protocatechuic acid, quercetin, riboflavin, rosmarinic acid, rutin, scopoletin, shikimic acid, thiamine, vitamin C, and β-carotene were purchased from Sigma-Aldrich (St. Louis, MO, USA) and used as external standards for the identification of compounds. Isotope-labeled L-[13C6] phenylalanine, used as an internal standard, was purchased from Sigma-Aldrich.

Preparation of standard solutions

The individual compounds were dissolved in methanol to a concentration of 100 µg/mL. Mixed working standard solutions were prepared at concentrations of 1 µg/mL and 0.01 µg/mL in ACN/IPA/water (3:3:2, v/v/v) from each stock solution. The stock solution of the internal standard was prepared by dissolving 0.4 mg labeled L-[13C6] phenylalanine in 1.0 mL of ACN/IPA/Water (3:3:2, v/v/v). All the standard solutions were stored in a refrigerator at -20 °C, and filtered through a 0.22-µm nylon syringe filter prior to analysis.

Preparation of sample solutions and quality controls

Noni juice samples (0.1 g) were accurately weighed into a 2-mL centrifuge tube and mixed with 1.5 mL of ACN/IPA/water (3:3:2, v/v/v) containing 10 µg/mL of internal standards (L-phenylalanine-13C6). The mixture was then vortexed for 5 min and sonicated for 1 h in an ice bath. After sonication, the mixture was centrifuged at 12,298 x g for 10 min at 4 °C, the supernatant was passed through a 0.22-µm nylon syringe filter (Whatman, Maidstone, UK). After that, 990 µL of supernatant was transferred to a new centrifuge tube and 10 µL of internal standard solution (0.4 mg/mL) was added before analysis. All samples were prepared in quintuplicate. Quality control (QC) samples (n = 4) were prepared by pooling equal volumes of an aliquot of each sample extract and analyzing every five samples of the run.

UHPLC-Q-TOF/MS analysis

The ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) system consisted of an Agilent 1260 Infinity II series (Agilent Technologies, Santa Clara, CA, USA) with a photodiode array (PDA) detector and an Agilent 6530 Q-TOF/MS (Agilent Technologies) equipped with an electrospray ionization (ESI) source. Chromatographic separation was performed on a YMC-Pack Pro C18 (150 × 4.6 mm, 3 μm) column (YMC Co., Kyoto, Japan). The mobile phase consisted of 0.1% formic acid in water (A) and 0.1% formic acid in ACN (B) with a gradient elution: 0–30 min, 1% B; 30–31 min, 40% B; 31–35 min, 100% B; 35–36 min, 1% B; 36–45 min, 1% B, at a flow rate of 0.3 mL/min. The temperature of the column oven was maintained at 30 °C and the injection volume was 10 µL. MS analysis was performed in both positive-ion and negative-ion modes using the MS and auto-MS/MS scan modes. The MS parameters were as follows: mass range, 25–1700 m/z; collision energy, 0, 10, and 20 V; gas temperature, 300 °C; drying gas, 10 L/min; nebulizer, 45 psi; sheath gas temperature, 300 °C; sheath gas flow, 11 L/min; capillary, 4,000 V; fragmentor, 175 V; and octapole RF Vpp, 750 V.

Data processing

Data acquisition and processing were performed using Agilent MassHunter Qualitative Analysis software (Version 10.0, Agilent Technologies). Raw MS data files acquired from the UHPLC-Q-TOF/MS analysis were identified using the METLIN database B 08.00, and their authentic compounds. The amount of each metabolite obtained by UHPLC-Q-TOF/MS was determined as the relative metabolite abundance, which was calculated by dividing all data values for each sample by the chromatographic peak area of the internal standards added to the metabolite extract of each sample.

Chemometric analysis

Principal component analysis (PCA), hierarchical clustering analysis (HCA), partial least–squares discriminant analysis (PLS-DA), and orthogonal partial least-squares discriminant analysis (OPLS-DA) were performed using the SIMCA-P software package (Umetrics, Umea, Sweden). Unsupervised data analysis, including PCA and HCA, was applied as an exploratory data analysis to visualize the analytical connections among the samples. PLS-DA and OPLS-DA were used for the modeling of samples in the retention of settled classes Y. In the PLS-DA output, the variable important in projection (VIP) is an important screening index of metabolites that changed between different non-juice samples. The PLS-DA models were also validated using R2Y and Q2 from a random permutation test (n = 200) in SIMCA-P. The quality of the PLS model was depicted by the cross-validation parameters R2 and Q2, which represent the explained variance and predictive capability of the model, respectively.

Results and discussion

Identification of bioactive compounds in noni juice and fermented noni juices

UHPLC-Q-TOF/MS was performed to analyze secondary metabolites in noni and fermented noni juice. As a result of our analysis, 130 compounds, including anthraquinones, coumarins, flavonoids, phenolic acids, phenolics, terpenoids, and miscellaneous com-pounds (acids, carbohydrates, vitamins, fatty acids, etc.) were tentatively identified from the samples (NJ1–NJ3) in both ESI + and ESI- modes (Fig. 1A and B). A total of 74, 83, and 91 compounds were detected in NJ1, NJ2, and NJ3, respectively. According to previous studies, anthraquinones, flavonoids, phenolic acids, phenolics, terpenoids, fatty acids, and carbohydrates are the major compounds in noni juice [15].

Fig. 1
figure 1

Classification information of differential metabolites identified between organic noni juice (NJ1) and fermented noni juices (NJ2 and NJ3) (A) and heatmap visualization of significant metabolites in positive mode (B). The horizontal bars of green, blue, and yellow describe the number of differential metabolites identified between noni juice and fermented noni juices. The color gradient of dark blue and deep red colors in the heat map indicates low and high intensities

Details of the 74 compounds identified in NJ1 are listed in Table S1. NJ1 included 9 anthraquinones (rubiadin 1-methyl ether, rhabarberone, emodin, 1,3-dihydroxy-2-methoxyanthracene-9,10-dione, 8-O-primeverose-1,3-dihydroxy-2-methyl-anthraquinone, 1-O-gentiobiose-2-methylolanthraquinone, 8-O-primeverose-1-methoxy-3-hydroxy-2-methyl-anthraquinone, 3-O-primeverose-1,6,8-trihydroxy-2-methyl-anthraquinone, and 1-O-gentiobiose-8-methoxy-aloeemodin), 4 coumarins (4-hydroxycoumarin, esculin, esculetin, and scopoletin), 14 flavonoids (isorhamnetin-3-O-galactoside, astragalin, epigallocatechin, quercetin-3-rhamnoside, D-catechin, rutin, isorhamnetin-3-O-rutinoside, quercetin, naringin, gallic acid, leptosin, hesperidin, kaempferol, and 2’,7-dihydroxy-4’,5’-dimethoxyisoflavone), 11 phenolic acids (3-hydroxyphenylpropionic acid, p-coumaric acid, 4-O-caffeoylquinic acid, cinnamic acid, caffeic acid, ferulic acid, salicylic acid, rosmarinic acid, hydroxybutanedioic acid, 2-hydroxy-2-phenylacetic acid, and chlorogenic acid), 10 phenolics (4-hydroxy-3-methoxystyrene, xanthoxylin, 4-hydroxy-3-methoxycinnamaldehyde, 3-hydroxybenzoic acid, nicotiflorin, protocatechuic acid, 3,4-dihydroxybenzaldehyde, 2,5-dihydroxybenzoic acid, 2-hydroxy-4-methylbenzaldehyde, and physcion), 12 terpenoids (8-acetylharpagide, asperulosidic acid, asperuloside, deacetylasperulosidic acid, monotropein, geniposidic acid, aucubin, harpagide acetate, geniposide, isoasperulosidic acid, rehmannioside A, and methoxygaertneroside), and 14 miscellaneous, such as acids, vitamins, and carbohydrates. A total of 83 compounds were detected in NJ2, including 12 anthraquinones, four coumarins, 16 flavonoids, 14 phenolic acids, 11 phenolics, 13 terpenoids, and 13 miscellaneous compounds (Table SS2). More flavonoids (16 compounds) and phenolic acids (14 compounds) were detected in NJ2 than those in NJ1 (14 flavonoids and 11 phenolic acids). In contrast, 91 compounds were putatively identified in NJ3. NJ3 contains 5 anthraquinones, 5 coumarins, 33 flavonoids, 13 phenolic acids, 10 phenolics, 12 terpenoids, and 13 miscellaneous compounds (Table SS3). The levels of the detected bioactive compounds tended to increase during fermentation. Eleven phenolic acids were detected in NJ1, whereas 14 and 13 were detected in NJ2 and NJ3, respectively. Fourteen flavonoids were identified in NJ1, and 16 and 33 flavonoids were identified in NJ2 and NJ3, respectively. In particular, astragalin, a 3-O-glucoside of kaempferol, was detected exclusively in NJ1, while kaempferol, the aglycone form of astragalin, was found in the post-fermentation sample (NJ2 and NJ3). It is widely acknowledged that aglycones demonstrate higher activity within the intestine compared to their glycoside counterparts, resulting in enhanced bioavailability [16]. In addition, it has been reported that LAB fermentation can cause deglycosylation from flavonoids [17]. Three more phenolic acids (3,5-O-dicaffeoylquinic acid, ferulic acid derivative, and sinapic acid) were found in NJ2 compared with NJ1. In the previous study, fermentation by LAB increased the total phenolic content and the antioxidant capacity in jujube-wolfberry composite juice [18]. Therefore, these results suggest that LAB fermentation could have a positive effect on the metabolite changes in noni fruit. On the other hand, the number of flavonoids was considerably higher in NJ3 than that in NJ1 or NJ2, which seemed to be derived from calamansi (Citrus microcarpa) juice in NJ3. Calamansi is a small citrus fruit that contains the highest amount of phenolic acids, p-coumaric acids, and flavonoids [19]. In this study, citrus flavonoids, such as naringenin, neodiosmin, nobiletin, neohesperidin, and tangeretin, were exclusively found in NJ3. Noni fruits have been reported to contain large amounts of iridoid compounds, among which deacetylasperulosidic acid, asperulosidic acid, and asperuloside are the major iridoids [20]. Coumarins play important roles in regulating plant growth and metabolites [21]. They also exhibit a wide range of biological functions, including anti-inflammatory, antioxidant, antibacterial, and anticancer [22]. Among these, scopoletin is a representative coumarin derivative of noni fruit [23]. In this study, all samples contained major iridoid compounds such as deacetylasperulosidic acid, asperulosidic acid, and asperuloside.

Metabolomic profiles of different noni juice samples

Untargeted UHPLC-Q-TOF/MS chemical fingerprinting coupled with chemometric analysis was performed to determine the comprehensive metabolite profiles of the different noni juice samples. The normalized data of NJ1, NJ2, and NJ3 in both positive and negative modes for both UHPLC-Q-TOF/MS are shown in Fig. 1. After data normalization, unsupervised PCA and HCA were performed using the normalized data of different noni juice samples to reveal the same sample clustering pattern, obtain an overview of the trend, and determine putative outliers (Fig. 2A and B). The PCA score plot showed a clear separation according to the three groups of noni juice samples including ‘NJ1,’ ‘NJ2,’ and ‘NJ3’ with no significant sample outliers. The plot was built using the first two principal components (PCs), PC1 and PC2, of the total variance (PC1 = 40.7% and PC2 = 34.1%). HCA was performed to elucidate the similarities and dissimilarities between the metabolite profiles of different samples. The HCA dendrogram indicated a clear separation between the non-fermented juice group (Group 1) and the fermented juice group (Group 2). These results imply that the major metabolite profiles of noni juice may be affected mainly by fermentation with the mixture of probiotics used in this study.

Supervised PLS-DA was performed to calculate models that differentiated between groups and to select the metabolites responsible for the different noni juice sample-dependent clustering based on VIP values > 1. The PLS-DA score plot showed clear separation of the three clusters representing the different noni juice samples (Fig. 2C). The observed PLS-DA model had good coefficient fractions (p < 0.05), with R2Y = 0.989 and Q2 = 0.979 (positive predictive ability), and the variables explained 0.749 (R2X) of the total variation. The y intercepts of R2 and Q2 in the permutation test were 0.187 and − 0.398, respectively, indicating a valid model (Fig. 2D). The recommended values for good fitting of models have been described as R2Y intercept < 0.3 and Q2Y intercept < 0.05. Differential metabolites were selected according to the criteria of p < 0.05 and VIP score > 1 from the PLS-DA model (Table 1). Hence, a combination of chromatographic fingerprints based on differential metabolite profiling with PLS-DA provides more comprehensive and insightful information regarding the dissimilarities between different noni juice samples.

Fig. 2
figure 2

Unsupervised score plots of the PCA model (A) and HCA dendrogram (B) and supervised score plot of the PLS-DA model (C). Two hundred permutation test (D) on PLS model from chemical fingerprinting of three noni juices

Table 1 Variable importance in projection (VIP) scores of partial least squares-discriminant analysis (PLS-DA)

Investigation of the OPLS-DA score plot and S-line plots was conducted to identify peaks that differed by sample type (Fig. 3). Within the S-line plots, iridoid compounds, flavonoids, and sucrose that showed substantial differences between each noni juice sample by matching them with an in-house library (Table SS4). In comparison between NJ1 and NJ2, the contents of asperulosidic acid (m/z 431.1202 [M ̶H]¯), isoasperulosidic acid (m/z 431.1199 [M ̶H]¯), and rutin (m/z 611.1612 [M + H]+) were higher in NJ1 than NJ2, whereas the contents of deacetylasperulosidic acid (m/z 389.1099 [M ̶H]¯) and monotropein (m/z 391.1234 [M + H]+) from NJ2 were higher than those from NJ1. Similarly, NJ1 had higher asperulosidic acid, and isoasperulosidic acid contents in NJ1 than those in NJ2. A previous study reported that the deacetylasperulosidic acid content was higher in fermented Morinda citrifolia L. extract (MCE) than that in non-fermented MCE, whereas the asperulosidic acid content was lower in fermented MCE than that in non-fermented extract [24]. These results imply that asperulosidic acid may be acetylated during noni juice fermentation. A previous study also reported that an increase in the contents of polysaccharides, free anthraquinones, rubiadin, monotropein, and fructose in noni juice fermented by Bacillus sp. DU-106 and Lactobacillus plantarum [24]. Dendrobium officinale fermented by Bacillus sp. DU-106 showed enhanced the immunostimulatory activity [26]. In addition, monotropein induced immune activation in NCM460 cells [27]. Noni juice fermented by LAB showed the enhancement of immune activities compared to non-fermented noni juice [28]. It appears that changes in metabolite in noni juice caused by LAB fermentation could affect immune activity of noni juice.

Fig. 3
figure 3

OPLS-DA score plot and S-line plots of the non-fermented noni juice (NJ1) and the fermented noni juice (NJ2) (A, D; R2X = 0.723, R2Y = 0.992, Q2 = 0.985), the non-fermented noni juice (NJ1) and the fermented noni juice added with organic coconut blossom sugar, calamansi juice and organic maltodextrin (NJ3) (B, E; R2X = 0.686, R2Y = 0.991, Q2 = 0.987), and the the only fermented noni juice (NJ2) and the fermented noni juice added with the supplementary materials (NJ3) (C, F; R2X = 0.689, R2Y = 0.999, Q2 = 0.976)

Noni fruits are typically fermented before consumption due to their unpleasant odors and taste [29]. In addition, commercially available noni products are processed in various ways by adding sugar, fruits, and condiments to reduce undesirable odor [30]. Citrus fruits, which contain linalool and limonene—very floral and strong odors—can be added as additives to noni juices to mask flavor or eliminate odor [31]. Adding fruit juices to noni juice is considered a convenient way to create value-added fruit drinks that excel in both sensory and nutritional quality. In this study, NJ3, made by adding coconut pollen sugar, calamansi juice, and maltodextrin, was used as a fermented noni juice to reflect commercial blended noni juice. NJ3 had higher contents of 3,5,7,4ʹ-tetramethoxyflavone (m/z 341.1055 [M ̶H]¯), 5-hydroxy-6,7,8,3ʹ,4ʹ-pentamethoxyflavone (m/z 373.1284 [M ̶H]¯), and sucrose (m/z 341.1093 [M ̶H]¯) than those in NJ1 and NJ2 (Table SS4). Coconut blossom sugar is produced from the phloem sap of the blossom of the coconut palm tree (Cocos nucifera L.), which is heated until it begins to form a thick syrup [32]. Sugar contains little fructose and has a lower glycemic index than conventional refined cane or beet sugars [33]. Citrus fruits such as calamansi are rich sources of polymethoxylated flavones that could be responsible for their antioxidant, anti-inflammatory, and antiviral potentials [34,35,36,37]. The high polymethoxyflavone and sucrose contents in NJ3 could be attributed to organic coconut blossom sugar and calamansi juice, respectively. However, significant variations in metabolite profiles are expected based on the specific ingredients added to noni juice, indicating the need for further research on metabolites in commercial noni juice formulations.

In conclusion, we confirmed the metabolic profiles of noni fruit juice and fermented noni fruit juices by using UHPLC-Q-TOF/MS. A total of 74, 83, and 91 compounds were identified in NJ1, NJ2, and NJ3, respectively. Untargeted UHPLC-Q-TOF/MS chemical fingerprinting coupled with chemometric analysis showed the comprehensive metabolite profiles of the different noni juice samples. These results can be used as basic information for developing products with fermented noni juice. Finally, further research is ongoing to improve the quality of noni juice according to fermentation conditions.

Data availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Abbreviations

LAB:

Lactic acid bacteria

NJ1:

Organic noni fruit juice

NJ2:

Fermented organic noni fruit juice I

NJ3:

Fermented organic noni fruit juice II

ACN:

Acetonitrile

IPA:

Isopropanol

QC:

Quality control

UHPLC-Q-TOF/MS:

Ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry

PCA:

Principal component analysis

HCA:

Hierarchical clustering analysis

PLS-DA:

Partial least-squares discriminant analysis

OPLS-DA:

Orthogonal partial least-squares discriminant analysis

References

  1. Dussossoy E, Brat P, Bony E, Boudard F, Poucheret P, Mertz C, Giaimis J, Michel A (2011) Characterization, anti-oxidative and anti-inflammatory effects of Costa Rican noni juice (Morinda citrifolia L). J Ethnopharmacol 133:108–115

    Article  CAS  PubMed  Google Scholar 

  2. Deng S, Palu K, West BJ, Su CX, Zhou BN, Jensen JC (2007) Lipoxygenase inhibitory constituents of the fruits of noni (Morinda citrifolia) collected in Tahiti. J Nat Prod 70:859–862

    Article  CAS  PubMed  Google Scholar 

  3. Palu AK, Kim AH, West BJ, Deng S, Jensen J, White L (2008) The effects of Morinda citrifolia L. (noni) on the immune system: its molecular mechanisms of action. J Ethnopharmacol 115:502–506

    Article  CAS  PubMed  Google Scholar 

  4. Wang MY, West BJ, Jensen CJ, Nowicki D, Su C, Palu AK, Anderson G (2002) Morinda citrifolia (Noni): a literature review and recent advances in Noni research. Acta Pharmacol Sin 23:1127–1141

    CAS  PubMed  Google Scholar 

  5. Potterat O, Felten RV, Dalsgaard PW, Hamburger M (2007) Identification of TLC markers and quantification by HPLC-MS of various constituents in noni fruit powder and commercial noni-derived products. J Agric Food Chem 55:7489–7494

    Article  CAS  PubMed  Google Scholar 

  6. Pawlus AD, Kinghorn DA (2007) Review of the ethnobotany, chemistry, biological activity and safety of the botanical dietary supplement Morinda citrifolia (noni). J Pharm Pharmacol 59:1587–1609

    Article  CAS  PubMed  Google Scholar 

  7. Mohd Zin Z, Abdul Hamid A, Osman A, Saari N, Misran A (2007) Isolation and identification of antioxidative compound from fruit of Mengkudu (Morinda citrifolia L). Int J Food Prop 10:363–373

    Article  Google Scholar 

  8. Gong X, Jiang S, Tian H, Xiang D, Zhang J (2020) Polyphenols in the fermentation liquid of Dendrobium candidum relieve intestinal inflammation in zebrafish through the intestinal microbiome-mediated immune response. Front Immunol 11:1542

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Deng S, West BJ, Jensen CJ (2010) A quantitative comparison of phytochemical components in global noni fruits and their commercial products. Food Chem 122:267–270

    Article  CAS  Google Scholar 

  10. Yadav H, Jain S, Rastamanesh R, Bomba A, Catanzaro R, Marotta F (2012) Fermentation technology in the development of functional foods for human health: where we should head. Ferment Technol 1:e102. https://doi.org/10.4172/2167-7972.1000e102

    Article  PubMed  PubMed Central  Google Scholar 

  11. Rein MJ, Renouf M, Cruz-Hernandez C, Actis-Goretta L, Thakkar SK, da Silva Pinto M (2013) Bioavailability of bioactive food compounds: a challenging journey to bioefficacy. Br J Clin Pharmacol 75:588–602

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Chen W, Zhu J, Niu H, Song Y, Zhang W, Chen H, Chen W (2018) Composition and characteristics of yam juice fermented by Lactobacillus plantarum and Streptococcus thermophilus. Int J Food Eng 14:20180123. https://doi.org/10.1515/ijfe-2018-0123

    Article  CAS  Google Scholar 

  13. Ricci A, Cirlini M, Calani L, Bernini V, Neviani E, Del Rio D, Galaverna G, Lazzi C (2019) In vitro metabolism of elderberry juice polyphenols by lactic acid bacteria. Food Chem 276:692–699

    Article  CAS  PubMed  Google Scholar 

  14. Bussmann RW, Hennig L, Giannis A, Ortwein J, Kutchan TM, Feng X (2013) Anthraquinone content in Noni (Morinda citrifolia L.). Evidence-Based Complementary Altern Med 2013:208378. https://doi.org/10.1155/2013/208378

  15. Zhang B, Zhou XM, Zhao T, Wang JL, Li MM, Lyu S, Zhou Q, Chen GY (2019) Chemical constituents from fermented Noni juice. Zhongguo Zhong Yao Za Zhi 44:4015–4020

    PubMed  Google Scholar 

  16. Day AJ, Canada FJ, Diaz JC, Kroon PA, McLauchlan R, Faulds CB, Plumb GW, Morgan MR, Williamson G (2000) Dietary flavonoid and isoflavone glycosides are hydrolysed by the lactase site of lactase phlorizin hydrolase. FEBS Lett 468:166–170

    Article  CAS  PubMed  Google Scholar 

  17. Marazza JA, Garro MS, de Giori GS (2009) Aglycone production by Lactobacillus rhamnosus CRL981 during soymilk fermentation. Food Microbiol 26:333–339

    Article  CAS  PubMed  Google Scholar 

  18. Zhao X, Tang F, Cai W, Peng B, Zhang P, Shan C (2023) Effect of fermentation by lactic acid bacteria on the phenolic composition, antioxidant activity, and flavor substances of jujube–wolfberry composite juice. LWT 184:114884

    Article  CAS  Google Scholar 

  19. Cheong MW, Chong ZS, Liu SQ, Zhou W, Curran P, Bin Y (2012) Characterisation of calamansi (Citrus microcarpa). Part I: volatiles, aromatic profiles and phenolic acids in the peel. Food Chem 134:686–695

    Article  CAS  PubMed  Google Scholar 

  20. Deng S, West BJ, Palu K, Jensen CJ (2011) Determination and comparative analysis of major iridoids in different parts and cultivation sources of Morinda citrifolia. Phytochem Anal 22:26–30

    Article  CAS  PubMed  Google Scholar 

  21. Robe K, Izquierdo E, Vignols F, Rouached H, Dubos C (2021) The coumarins: secondary metabolites playing a primary role in plant nutrition and health. Trends Plant Sci 26:248–259

    Article  CAS  PubMed  Google Scholar 

  22. Zhu JJ, Jiang JG (2018) Pharmacological and nutritional effects of natural coumarins and their structure-activity relationships. Mol Nutr Food Res 62:1701073

    Article  Google Scholar 

  23. Wu L, Wang X, Xu W, Farzaneh F, Xu R (2009) The structure and pharmacological functions of coumarins and their derivatives. Curr Med Chem 16:4236–4260

    Article  CAS  PubMed  Google Scholar 

  24. Choi SI, Kwon HY, La IJ, Jo YH, Han X, Men X, Lee SJ, Kim YD, Seong GS, Lee OH (2021) Development and validation of an analytical method for deacetylasperulosidic acid, asperulosidic acid, scopolin, asperuloside and scopoletin in fermented Morinda citrifolia L.(Noni). Separations 8:80

    Article  CAS  Google Scholar 

  25. Tan LR, Dai WH, Luo ZF, Li WZ, Li P, Lin WW, Cheng M, Du B (2018) Effect of fermentation with different strains on active components content in Morinda officinalis how. China Brew 37:121–125

    Google Scholar 

  26. Tian W, Dai L, Lu S, Luo Z, Qiu Z, Li J, Li P, Du B (2019) Effect of Bacillus sp. DU-106 fermentation on Dendrobium officinale polysaccharide: structure and immunoregulatory activities. Int J Biol Macromol 135:1034–1042

    Article  CAS  PubMed  Google Scholar 

  27. Qiang J, Yang R, Li X, Xu X, Zhou M, Ji X, Lu Y, Dong Z (2024) Monotropein induces autophagy through activation of the NRF2/PINK axis, thereby alleviating sepsis-induced colonic injury. Int Immunopharmacol 127:111432

    Article  CAS  PubMed  Google Scholar 

  28. Choi SI, Han X, Men X, Lee SJ, Kim YD, La IJ, Seong GS, Lee OH (2022) Enhancement of immune activities of fermented Morinda citrifolia L. (noni) and six marker compounds. J Food Hyg Saf 37:29–37

    Article  Google Scholar 

  29. Wang Z, Dou R, Yang R, Cai K, Li C, Li W (2021) Changes in Phenols, Polysaccharides and volatile profiles of Noni (Morinda citrifolia L.) Juice during Fermentation. Molecules 26:2604

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Ali M, Kenganora M, Manjula S (2016) Health benefits of Morinda citrifolia (Noni): a review. Pharmacognosy J 8:321–334

    Article  CAS  Google Scholar 

  31. Mostafa S, Wang Y, Zeng W, Jin B (2022) Floral scents and Fruit aromas: functions, compositions, biosynthesis, and Regulation. Front Plant Sci 13:860157

    Article  PubMed  PubMed Central  Google Scholar 

  32. Saraiva A, Carrascosa C, Ramos F, Raheem D, Lopes M, Raposo A (2023) Coconut sugar: chemical analysis and nutritional profile; health impacts; safety and quality control; food industry applications. Int J Environ Res Public Health 20:3671

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Wrage J, Burmester S, Kuballa J, Rohn S (2019) Coconut sugar (Cocos nucifera L.): production process, chemical characterization, and sensory properties. LWT 112:108227. https://doi.org/10.1016/j.lwt.2019.05.125

    Article  CAS  Google Scholar 

  34. Singh B, Singh JP, Kaur A, Singh N (2020) Phenolic composition, antioxidant potential and health benefits of citrus peel. Food Res Int 132:109114. https://doi.org/10.1016/j.foodres.2020.109114

    Article  CAS  PubMed  Google Scholar 

  35. Xu JJ, Wu X, Li MM, Li GQ, Yang YT, Luo HJ, Huang WH, Chung HW, Ye WC, Wang GC, Li YL (2014) Antiviral activity of polymethoxylated flavones from Guangchenpi, the edible and medicinal pericarps of citrus reticulata ‘Chachi’. J Agric Food Chem 62:2182–2189

    Article  CAS  PubMed  Google Scholar 

  36. Chen XM, Tait AR, Kitts DD (2017) Flavonoid composition of orange peel and its association with antioxidant and anti-inflammatory activities. Food Chem 218:15–21

    Article  CAS  PubMed  Google Scholar 

  37. Fu M, Xu Y, Chen Y, Wu J, Yu Y, Zou B (2017) Evaluation of bioactive flavonoids and antioxidant activity in Pericarpium Citri Reticulatae (Citrus reticulata ‘Chachi’) during storage. Food Chem 230:649–656

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

Not applicable.

Funding

This research was supported by Atomy Orot Co., LTD and NSTBio Co., LTD, Korea Basic Science Institute (National research Facilities and Equipment Center) grant funded by the Ministry of Education (2019R1A6C1010044), and the BB21plus funded by Busan Metropolitan City and Busan Techno Park.

Author information

Authors and Affiliations

Authors

Contributions

Yoonjeong Kim contributed to the data acquisition, data analysis and paper writing. JP and KK contributed to the data acquisition. JYL, EMK, IJL, and OHL contributed to conception and design of study. JS and Younghwa Kim contributed to the analysis of data and paper revision. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Jeehye Sung or Younghwa Kim.

Ethics declarations

Competing interests

The authors declare that they have no competing interests.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary Material 1

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kim, Y., Pyeon, J., Lee, JY. et al. Chemical fingerprint analysis of fermented Morinda citrifolia L. (Noni) juice by UHPLC Q-TOF/MS combined with chemometric analysis. Appl Biol Chem 67, 59 (2024). https://doi.org/10.1186/s13765-024-00910-w

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/s13765-024-00910-w

Keywords