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Phototactic behavioral response of agricultural insects and stored-product insects to light-emitting diodes (LEDs)
Applied Biological Chemistry volume 60, pages 137–144 (2017)
Abstract
Agricultural insects and stored-product insects are influenced by luminance intensities, exposure times, and wavelengths of light-emitting diodes (LEDs). Based on the phototactic behaviors of the agricultural insects, green or blue LEDs are most attractive for Bemisia tabaci, Trialeurodes vaporariorum, Myzus persicae, Liriomyza trifolii, Spodoptera exigua, and Spodoptera litura. Green LED attracts Plutella xylostella and Frankliniella occidentalis. Similarly, green or blue LEDs are more attractive to agricultural insects, such as Liriomyza sativae, Sogatella furcifera, and Nilaparvata lugens, than other wavelength LEDs. Concerning the phototactic behaviors of the stored-product insects, red LED is attractive for, in descending order Tribolium castaneum, Sitophilus zeamais, Lasioderma serricorne, and Tyrophagus putrescentiae. Blue LED captures most Sitophilus oryzae and Sitotroga cerealella. Red and blue LEDs are more attractive for stored-product insect pests rate than ultraviolet LED and green, yellow, white, and infrared LEDs. Based on the attraction rate of the stored-product insects on granary, red LED is most attractive for S. cerealella and Plodia interpunctella. These light sources are effective in controlling agricultural and stored-product insects. Applying LED technology for greenhouses and granaries along with conventional traps reduces crop loss due to moths, beetles, aphids, and weevils. LEDs have potential value in integrated pest management.
Introduction
Many countries are using synthetic insecticides as the primary means of controlling insect pests [1, 2]. However, repeated use of synthetic insecticides can increase the development of resistance in the insect pests and has negative effects on the environment and nontarget insects [2, 3]. Efforts are ongoing to develop sustainable alternative and eco-friendly methods, such as the use of electric traps, food traps, and natural insecticides [1–4].
Phototaxis is the behavior of insect species in response to light sources. This movement is influenced by the light wavelength, and the quality and intensity of the light source [5]. In general, insect pests can perceive light ranging in wavelength from 350 to 700 nm and respond in diverse ways [6]. The alternative techniques being developed include phototaxis; electric traps equipped with black and incandescent light bulbs are used for surveillance, for example. The incandescent bulb as the standard light commonly used in light traps ranges in wavelength from 350 to 700 nm with a maximum output wavelength at 700 nm [7]. Insect species can be attracted or repelled to special light sources, such as artificial lights [8]. Certain insect species exhibit a directional response to light-emitting sources including high- or low-intensity light [9]. The use of artificial light sources in integrated pest management (IPM) has increased globally [10]. Light-emitting diodes (LEDs) have emerged as an important technology in the development of agricultural systems [11, 12]. The many advantages of LEDs include the eco-friendly technology, functional improvement, plant growth, high luminous efficiency, selectivity of specific wavelength and light intensity, low weight, low electronic consumption, small size, prolonged lifetime, and environmental affinity [13, 14].
LED traps may be a potential alternative to commercial traps for mass trapping and phototactic monitoring of insect pests. Specific wavelength LED sources are being used for monitoring as well as trapping [7, 15]. Pest insects will move toward light lamps or other illuminations in outdoor settings [16]. This phototactic behavior of pest insects is the basis of the design of electronic insect traps [16]. The light traps are equipped with LED sources; they effectively attract agricultural and stored grain insect pests including aphids, beetles, moths, and weevils and prevent the entry of these insects into greenhouses and granaries [17, 18]. Interest is growing in control technology that exploits insect behaviors to light sources as an alternative to synthetic insecticides [16, 19]. Here, we review the advanced control technologies of insect species that employ new light sources including LEDs.
Classification of phototactic behavioral responses to light sources of various insects
Insect behavior to light is varied and can be categorized [20]. The typical behavior is phototaxis. Insects display several phototactic responses including attraction (movement toward the light source: positive phototaxis) and repulsion (movement away from the light source: negative phototaxis). Optimal conditions, which include effective wavelengths, exposure time, and intensities to light source, are diverse among insect species [21, 22]. Negative phototaxis could be useful to prevent entrance of pest insects to greenhouses and granaries [23, 24]. Behavioral responses to light by insect species also include light adaptation, circadian periodicity, photoperiodism, and light toxicity [20]. Nocturnal insects can adapt to light sources; typical adaptive behaviors are diminished migration, settling near the light source, and mating [20]. Circadian periodicity is daily behavioral response that encompasses courtship, feeding, flight, and locomotion [25]. Artificial light at night can change the diurnal or nocturnal responses and timing of insect species [26], which represents a phase shift in chronobiology [27]. Photoperiodism is the physiological behavior of insect species to light, such as day light. The start of resting can be prevented by repeatedly exposing insects to light sources for some days [28]. Insects that do not enter diapause cannot overwinter. Continuous light irradiation is structurally damaging and causes light toxicity [29]. Photo-irradiation is also useful for treatment of crops before the post-harvest in the greenhouse and granary settings. Insect behaviors to light sources are significantly influenced by various factors of the light, such as intensity, single or combined wavelengths, exposure time, and differences of light intensity and color to those of ambient lighting [19, 30]. In the remainder of this minireview, we discuss the technologies being currently being used to control many insect species.
Phototactic behavior to LED source for agricultural insect pests
Agricultural insect species include aphids, leaf miners, moths, and whiteflies. Their reactions are influenced by various characteristics of light, such as luminance intensity, light exposure time, and light wavelength [31]. Evaluation of the phototactic responses of insect species to these aforementioned aspects typically uses a chamber capable of dark and illuminated settings (Figs. 1, 2). Phototactic behavior of agricultural insect species has been amply correlated with characteristics of light [11, 32–38]. Bemisia tabaci and Trialeurodes vaporariorum showed a significantly more favorable response to the green (520 nm) and blue (470 nm) LEDs at a luminance intensity of 40 lx and exposure time of 90 min than to red (625 nm) and yellow (590 nm) LEDs [34, 36]. Under optimal light exposure times and luminance intensities, LED light sources that emit relatively short wavelengths attract agricultural insect species [11, 32–38] (Table 1). Based on the phototactic behaviors, green and/or blue LEDs show the highest attraction rate against Bemisia tabaci (85.3 and 89.0%), Trialeurodes vaporariorum (96.6 and 97.4%), Myzus persicae (85.3 and 75.0%), Liriomyza trifolii (99.7 and 91.2%), Spodoptera exigua (90.3 and 81.1%), Spodoptera litura (64.3 and 57.7%), Plutella xylostella (green LED, 98.3%), and Frankliniella occidentalis (green LED, 44.3%) [11, 32–38]. In contrast, the relatively long wavelengths of red light (625 nm) and infrared light (IR, 730 nm) are repellent for the leaf miner (L. trifolii), moths (S. litura and P. xylostella), and whitefly (T. vaporariorum) [32, 33, 35, 36]. Green and/or blue LEDs are attractive for several agricultural insect pests including T. vaporariorum, Liriomyza sativae, Sogatella furcifera, and Nilaparvata lugens [39, 40]. Matteson and Terry [41] reported that F. occidentalis exhibited strong attractiveness to the blue LED traps. Vaishampayan et al. [42] evaluated the ultraviolet (UV, <400 nm), yellow-green region (520–610 nm), and red (610 to ca. 700 nm) light in attracting T. vaporariorum and found that the yellow-green region attracted the most individuals compared with ultraviolet and red light. The collective data indicate that light traps with green and blue LEDs have the potential to control the agricultural insects in IPM.
Phototactic behavior on stored-product insects to LED sources
Similar to the behaviors of agricultural insect species, the behavior of various stored-product insect pests, such as weevil and moth, are influenced by light sources [12, 15, 17, 43–46] (Fig. 3). Under optimal light exposure times and luminance intensities of each wavelength, red, green, and blue LEDs have proven to be most attractive for stored-product insect species [12, 17, 44–46]. Based on the phototactic behavior under laboratory conditions, red LED (625 nm) is more efficient in attracting Sitophilus zeamais (59.8%) and Tribolium castaneum (97.8%), but less efficient in attracting Lasioderma serricorne (31.0%) and Tyrophagus putrescentiae (18.0%) [24, 47] (Table 2). Blue LED is significantly more effective in attracting S. oryzae and Sitotroga cerealella than UV (365 nm), green (510–520 nm), red (625–660 nm), and IR (730 nm) LEDs [17, 44]. Green LED best attracted Plodia interpunctella (52.2%) [12]. Based on the attraction rate under optimal conditions (luminous intensity of 60 lx and exposure time of 4 days), red LED showed the highest attraction rate against S. cerealella (67.1%) and P. interpunctella (81.5%) [48, 49] in a granary setting (Table 3). In the same setting, P. interpunctella (66.3%) and S. cerealella (82.7%) exhibited strong attractiveness to the green and blue LEDs, respectively [48] (Fig. 4). The effectiveness of LEDs has also been chronicled for blossom weevil (Anthonomus pomorum) and sweet potato weevil (Euscepes postfasciatus) [15, 50]. Nakamoto and Kubo [15] reported that light trapping of E. postfasciatus was more efficient using green (536 nm) LED. Hausmann et al. [50] found that the green and blue LEDs were more efficient in attracting and trapping A. pomorum than UV light. These optimal conditions of LED sources are an advantage to control insect pest behaviors. In future, the development of LED devices containing practical application is expected.
Study on physical control of insect pests by using light sources
The influence of LEDs on insect color and light perception with different wavelengths and on behavior, and the development of control technology involving new light sources has been described [19]. Physiological systems have been comprehensively utilized to measure the influence on many insect species in a wide range of wavelengths [51]. Phototactic responses of many insect species to LED sources have been investigated to clarify the relationship between insect behaviors and light wavelengths, with the goal of determining the effective attractant and repellent wavelengths for target insect pests [51]. The development of LED sources that are able to be used instead of incandescent light traps is an ongoing research interest. In addition, wavelengths of light that effectively attract parasitoids, which are natural enemies of insect pests, are being investigated [19].
In conclusion, LED equipment with various wavelengths can now be manufactured due to current technological advances, and new agricultural technology using light is starting to attract attention. Advances are also expected in the use of light for insect control as the results of these technological developments in lighting. Based on the new research being conducted by National Agricultural Research Organisation, we hope to ensure the further development of agricultural technology founded on a good balance of input from basic study in universities and independent administrative institutions and applied technology from private companies and public research institutes to establish the next generation of pest control technology.
References
Lee HK, Lee HS (2016) Toxicities of active constituent isolated from Thymus vulgaris flowers and its structural derivatives against Tribolium castaneum (Herbst). Appl Biol Chem 59:821–826
Kim MG, Lee HS (2016) Insecticidal toxicities of naphthoquinone and its structural derivatives. Appl Biol Chem 59:3–8
Lee HW, Lee HS (2015) Acaricidal potency of active constituent isolated from Mentha piperita and its structural analogs against pyroglyphid mites. J Korean Soc Appl Biol Chem 58:597–602
Yun JH, Kim YJ, Koh KH (2016) Investigation into factors influencing antioxidant capacity of vinegars. Appl Biol Chem 59:495–509
Cloyd RA (2007) Phototaxis of fungus gnat, Bradysia sp. nr coprophila (Lintner) (Diptera: Sciaridae), adults of different light intensities. HortScience 42:1217–1220
Land MF (1997) Visual acuity in insects. Annu Rev Entomol 42:147–177
Bishop AL, Worrall R, Spohr LJ, McKenzie HJ, Barchi IM (2004) Response of Culicoides spp. (Diptera: Ceratopogonidae) to light-emitting diodes. Aust J Entomol 43:184–188
Vanninen I, Johansen NS (2005) Artificial lighting (AL) and IPM in greenhouse. IOBC/WPRS Bull 28:295–304
Turlings TCJ, Davison AC, Tamo C (2004) A six-arm olfactometer permitting simultaneous observation of insect attraction and odour trapping. Physiol Entonol 29:45–55
Garstang RH (2004) Mount Wilson observatory: the sad story of light pollution. Observatory 124:14–21
Oh MS, Lee CH, Lee SG, Lee HS (2011) Evaluation of high power light emitting diodes (HPLEDs) as potential attractants for adults Spodoptera exigua (Hubner) (Lepidoptera: Noctuidae). J Korean Soc Appl Biol Chem 54:416–422
Park JH, Lee HS (2016) Phototactic behavior 10: phototactic behavioral effects of Plodia interpunctella (Hűbner) (Lepidoptera: Pyralidae) adults to different light-emitting diodes of seven wavelengths. J Appl Biol Chem 59:95–98
Zheng LX, Zheng Y, Wu WJ, Fu YG (2014) Field evaluation of different wavelengths light-emitting diodes as attractants for adult Aleurodicus dispersus Russell (Hemiptera: Aleyrodidae). Neotrop Entomol 43:409–414
Yeh N, Chung JP (2009) High-brightness LEDs-energy efficient lighting sources and their potential in indoor plant cultivation. Renew Sustain Energy Rev 13:2175–2180
Nakamoto Y, Kuba H (2004) The effectiveness of a green light emitting diode (LED) trap at capturing the West Indian sweet potato weevil, Euscepes postfasciatus (Fairmaire) (Coleoptera: Curculionidae) in a sweet potato field. Appl Entomol Zool 39:419–495
Antignus Y (2000) Manipulation of wavelength-dependent behavior of insects: an IPM tool to impede insects and restrict epidemics of insect-borne viruses. Virus Res 71:213–220
Kim MG, Lee HS (2014) Phototactic behavior 5: attractive effects of the Angoumois grain moth, Sitotroga cerealella, to light-emitting diodes. J Korean Soc Appl Biol Chem 57:259–262
Park JH, Lee SM, Lee SG, Lee HS (2014) Attractive effects efficiency of LED trap on controlling Plutella xylostella adults in greenhouse. J Appl Biol Chem 57:255–257
Honda K (2011) Reactions to light in insects and practical applications. J Soc Biochem 35:233–236
Shimoda M, Honda K (2013) Insect reactions to light and its applications to pest management. Appl Entomol Zool 48:413–421
Kinoshita M, Arikawa K (2000) Colour constancy in the swallowtail butterfly Papilio xuthus. J Exp Biol 203:3521–3530
Yang EC, Lee DW, Wu WY (2003) Action spectra of phototactic responses of the flea beetle, Phyllotreta striolata. Physiol Entomol 28:362–368
Reisenman CE, Lazzari CR, Giurfa M (1998) Circadian control of photonegative sensitivity in the haematophagous bug Triatoma infestans. J Com Physiol A 183:533–541
Kim MG, Yang JY, Lee HS (2013) Phototactic behavior: repellent effects of cigarette beetle, Lasioderma serricorne (Coleoptera: Anobiidae), to light-emitting diodes. J Appl Biol Chem 56:331–333
Shimoda M, Kiguchi K (1995) The sweet potato hornworm, Agrius convolvuli, as a new experimental insect: behavior of adult moths in a rearing cage. Jpn J Appl Entomol Zool 39:321–328
Okada Y, Tomioka K, Chiba Y (1991) Circadian phase-response curves for light in nymphal and adult crickets, Gryllus bimaculatus. J Insect Physiol 37:583–590
Pittendrigh CS (1993) Temporal organization: reflections of a Darwinian clock-watcher. Annu Rev Physiol 55:17–54
Saunders D (2012) Insect photoperiodism: seeing the light. Physiol Entomol 37:207–218
Ghanem I, Shamma M (2007) Effect of non-ionizing radiation (UVC) on the development of Trogoderma granarium Everts. J Stored Prod Res 43:362–366
Nissinen A, Kristoffersen L, Anderbrant O (2008) Physiological state of female and light intensity affect the host-plant selection of carrot phyllid, Trioza apicalis (Hemiptera: Triozidae). Eur J Entomol 105:227–232
Sambaraju KR, Phillips TW (2008) Responses of adult Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae) to light and combinations of attractants and light. J Insect Behav 21:422–439
Cho KS, Lee HS (2012) Visual preference of diamondback moth, Plutella xylostella, to light-emitting diodes. J Korean Soc Appl Biol Chem 55:681–684
Yang JY, Kim MG, Hs Lee (2012) Phototactic behavior: attractive effects of Spodoptera litura (Lepidoptera: Noctuidae), tobacco cutworm, to high-power light-emitting diodes. J Korean Soc Appl Biol Chem 55:809–811
Kim MG, Yang JY, Chung NH, Lee HS (2012) Photo-response of tobacco whitefly, Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae), to light-emitting diodes. J Korean Soc Appl Biol Chem 55:567–569
Kim MG, Lee HS (2014) Attractive effects of American serpentine leafminer, Liriomyza trifolii (Burgess), to light-emitting diodes. J Insect Behav 27:127–132
Jeon JH, Kim MG, Lee HS (2014) Phototactic behavior 4: attractive effects of Trialeurodes vaporariorum adults to light-emitting diodes under laboratory conditions. J Korean Soc Appl Biol Chem 57:197–200
Yang JY, Lee SM, Lee HS (2015) Phototactic behavior 6: behavioral responses of Myzus persicae (Hemiptera: Aphididae) to light-emitting diodes. J Korean Soc Appl Biol Chem 58:9–12
Yang JY, Sung BK, Lee HS (2015) Phototactic behavior 8: phototactic behavioral responses of western flower thrips, Frankliniella occidentalis Pergande (Thysanoptera: Thripidae), to light-emitting diodes. J Korean Soc Appl Biol Chem 58:359–363
Yang HB, Hu G, Zhang G, Chen X, Zhu ZR, Liu S, Liang ZL, Zhang XX, Cheng XN, Zhai BP (2013) Effect of light colours and weather conditions on captures of Sogatella furcifera (Horváth) and Nilaparvata Lugens (Stål). J Appl Entomol 138:743–753
Affeldt HA, Thimijan RW, Smith FF, Webb RE (1983) Response of the greenhouse whitefly (Homoptera: Aleyrodidae) and the vegetable leafminer (Diptera: Agromyzidae) to photospectra. J Econ Entomol 76:1405–1409
Matteson NA, Terry LI (1992) Response to color by male and female Frankliniella occidentalis during swarming and non-swarming behavior. Entomol Exp Appl 63:187–201
Vaishampayan M, Kogan M, Waldbauer GP, Woolley JT (1975) Spectral specific responses in the visual behavior of the greenhouse whitefly, Trialeurodes vaporariorum (Homoptera: Aleyrodidae). Entomol Exp Appl 18:344–356
Thomas C, Gries G (2009) Ultraviolet and violet light: attractive orientation cues for the Indian meal moth, Plodia interpunctella. Entomol Exp Appl 131:148–158
Jeon JH, Oh MS, Cho KS, Lee HS (2012) Phototactic responses of the rice weevil, Sitophilus oryzae Linnaeus (Coleoptera: Curculionidae), to light-emitting diodes. J Korean Soc Appl Biol Chem 55:35–39
Park JH, Sung BK, Lee HS (2015) Phototactic behavior 7: phototactic response of the maize weevil, Sitotroga zeamais motsch (Coleopter: Curculionidae), to light-emitting diodes. J Korean Soc Appl Biol Chem 58:373–376
Song J, Jeong EY, Lee HS (2016) Phototactic behavior 9: phototactic behavioral response of Tribolium castaneum (Herbst) to light-emitting diodes of seven different wavelengths. J Appl Biol Chem 59:99–102
Lee SM, Lee JB, Lee HS (2015) Controlling Tyrophagus putrescentiae Adults in LED-equipped Y-maze chamber. J Appl Biol Chem 58:101–104
Jeon YJ, Lee HS (2016) Control effects of LED trap to Sitotroga cerealella and Plodia interpunctella in the granary. J Appl Biol Chem 59:203–206
Song J, Jeong EY, Lee HS (2016) Effect of LED trap on controlling Sitophilus zeamais and Tribolium castaneum in granary. J Appl Biol Chem 59:129–132
Hausmann C, Samietz J, Dorn S (2004) Visual orientation of overwintered Anthonomus pomorum (Coleoptera: Curculionidae). Environ Entomol 33:1410–1415
Hironaka M, Hariyama T (2009) Insect orientation to natural and artificial light. Jpn J Appl Entomol Zool 53:135–145
Acknowledgments
This work was carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project title: Development of integrated pest management techniques using natural products and LEDs in the grain storage, Project No. PJ01004501)” Rural Development Administration, Korea.
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Park, JH., Lee, HS. Phototactic behavioral response of agricultural insects and stored-product insects to light-emitting diodes (LEDs). Appl Biol Chem 60, 137–144 (2017). https://doi.org/10.1007/s13765-017-0263-2
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DOI: https://doi.org/10.1007/s13765-017-0263-2